Tgfbr1 Knockout mouse models
Tgfbr1 encodes ALK5, ortholog of human TGFBR1, a type I serine and threonine kinase receptor recruited into a heterotetramer by ligand-bound TGFBR2, which transphosphorylates the glycine and serine rich GS box; FKBP12 occupies that box in the basal state to prevent leaky activation, and once released the kinase phosphorylates SMAD2 and SMAD3 at their carboxy-terminal motifs while also engaging TRAF6, TAK1 and p38 for non-SMAD output. Heterozygous kinase domain mutations cause Loeys-Dietz syndrome with aortic aneurysm, so patient knockins address vascular biology that deletion does not. Germline deletion is embryonic lethal from yolk sac vasculogenesis failure, mandating conditional nulls for fibrosis, immune tolerance and tumor microenvironment studies, while a constitutively active T204D knockin driven from a lox-stop-lox cassette tests sufficiency. Because ALK1 and ALK2 transduce distinct ligand subsets, do not assume ALK5 loss removes all superfamily input from a tissue.
A conventional Tgfbr1 knockout removes gene function in all cells that inherit the allele. It is a proven first pass for target validation when redundancy is low.
What Tgfbr1 Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct Tgfbr1 knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Tgfbr1-KO | Knockout | KO/CKO mice | embryo cryopreservation | KO 225082 | Inquire |
Why this approach
A conventional knockout answers whether the gene is required broadly. When your target tissue is the organ of interest, a global null can still be informative if viability is acceptable and you want the simplest genotype. If the null is harsh, a floxed allele with a regional Cre is the safer long term platform.
Pricing and quotes
The Tgfbr1 Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Tgfbr1 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Tgfbr1 Knockout mouse models are available?
When Tgfbr1 Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Tgfbr1 knockout embryonic lethal in mice?
It depends on background and allele design. Some Tgfbr1 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Tgfbr1 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Tgfbr1?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.