Tgfbr2 Conditional Knockout mouse models
Tgfbr2, the mouse ortholog of human TGFBR2, encodes the constitutively active type II serine/threonine kinase receptor that captures TGFB1, TGFB2, and TGFB3 and transphosphorylates the GS domain of the type I receptor ALK5 (Tgfbr1), licensing phosphorylation of the SMAD2 and SMAD3 carboxy-terminal SSXS motifs and SMAD4-dependent nuclear transcription. Human disease logic splits cleanly: biallelic frameshifts in the polyadenine BAT-RII tract drive microsatellite-unstable carcinomas, whereas heterozygous kinase-domain missense alleles cause Loeys-Dietz syndrome with paradoxically elevated SMAD2 output in aortic wall. Straight germline deletion is lethal near E10.5 from failed yolk sac vasculogenesis and hematopoiesis, so epithelial, fibroblast, or T cell questions require a floxed kinase-domain allele driven by a validated lineage Cre. Because ligand redundancy across three TGFB isoforms and intact activin and BMP arms (Acvr2a, Acvr2b, Bmpr2) preserve parallel SMAD1/5/8 flux, receptor deletion silences only the SMAD2/3 branch; reserve epitope humanization for ligand trap and antibody pharmacology.
A floxed Tgfbr2 allele paired with Cre gives spatial control that whole body knockouts cannot offer. People use it to separate developmental roles from adult homeostasis, to model somatic mutations, and to match disease that begins in one organ.
What Tgfbr2 Conditional Knockout mouse models are available?
ingenious targeting laboratory offers 2 distinct Tgfbr2 conditional knockout catalog mouse models. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Why this approach
Conditional knockout focuses the experiment on your target tissue while the rest of the animal keeps a wild type allele. That pattern mirrors somatic mutation in patients and avoids systemic compensation that can erase subtle phenotypes. It is often preferred when a germline null is lethal, weak, or confounded by developmental rescue.
Pricing and quotes
The Tgfbr2 Conditional Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Tgfbr2 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Tgfbr2 Conditional Knockout mouse models are available?
When Tgfbr2 Conditional Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Tgfbr2 knockout embryonic lethal in mice?
It depends on background and allele design. Some Tgfbr2 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Tgfbr2 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Tgfbr2?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.