Tigit Conditional Knockout mouse models
Tigit is the mouse ortholog of human TIGIT and encodes a single IgV domain inhibitory receptor bearing an ITIM and an ITT-like motif that is induced on activated and exhausted CD8 T cells, follicular helper cells, regulatory T cells, and NK cells, where it binds CD155 (Pvr) with high affinity and CD112 (Pvrl2) more weakly. Because CD226 engages the same ligands as a costimulatory receptor, TIGIT acts partly by outcompeting CD226 and disrupting its homodimerization, while cis interactions and Treg-intrinsic signaling shape ligand availability and IL-10 output. Design consequences are concrete: germline or conditional nulls test cell-intrinsic inhibition, ITIM or ITT-like knockins separate signaling from ligand sequestration, and ectodomain humanization is required whenever a clinical antibody with defined Fc effector function is evaluated, since Fc-dependent Treg depletion is a leading proposed mechanism. Cd96 and Cd226 modulate the same axis, so plan compound alleles with tumor control or Pdcd1 combination endpoints.
Tissue restricted knockout of Tigit keeps wild type function everywhere else. Breeding is often more robust, and the setup mirrors patient biology where mutations arise in a subset of cells.
What Tigit Conditional Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct Tigit conditional knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Tigit-Flox | Conditional Knockout | KO/CKO mice | sperm cryopreservation | CKO 2106293 | Inquire |
Why this approach
Conditional knockout focuses the experiment on your target tissue while the rest of the animal keeps a wild type allele. That pattern mirrors somatic mutation in patients and avoids systemic compensation that can erase subtle phenotypes. It is often preferred when a germline null is lethal, weak, or confounded by developmental rescue.
Pricing and quotes
The Tigit Conditional Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Tigit allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Tigit Conditional Knockout mouse models are available?
When Tigit Conditional Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Tigit knockout embryonic lethal in mice?
It depends on background and allele design. Some Tigit germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Tigit animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Tigit?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.