Tlr4 Knockout mouse models
Tlr4 is the mouse ortholog of human TLR4 and encodes the lipopolysaccharide receptor, which requires the accessory protein MD-2 (Ly96) to form a pocket accommodating five of the six lipid A acyl chains while CD14 and LBP deliver ligand, and dimerization then triggers MyD88 and TIRAP signaling at the plasma membrane followed by TRAM and TRIF dependent IRF3 activation after endocytosis. Species specificity is a first-order design issue, since mouse and human MD-2 and TLR4 differ in their response to underacylated lipid A species, so lipid A mimetics, monophosphoryl lipid A adjuvants, and antagonists such as eritoran behave differently, which is why humanized Tlr4 and Ly96 knockins are used for adjuvant and sepsis pharmacology. The spontaneous P712H allele and complete nulls are both viable, so conditional deletion in myeloid, endothelial, or intestinal epithelial compartments is the refinement step, with endotoxin shock, neuropathic pain, or steatohepatitis endpoints dictating allele choice.
A conventional Tlr4 knockout removes gene function in all cells that inherit the allele. It is a proven first pass for target validation when redundancy is low.
What Tlr4 Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct Tlr4 knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Tlr4-KO | Knockout | KO/CKO mice | live | KO 18052 | Inquire |
Why this approach
A conventional knockout answers whether the gene is required broadly. When your target tissue is the organ of interest, a global null can still be informative if viability is acceptable and you want the simplest genotype. If the null is harsh, a floxed allele with a regional Cre is the safer long term platform.
Pricing and quotes
The Tlr4 Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Tlr4 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Tlr4 Knockout mouse models are available?
When Tlr4 Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Tlr4 knockout embryonic lethal in mice?
It depends on background and allele design. Some Tlr4 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Tlr4 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Tlr4?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.