Tnf Conditional Knockout mouse — myeloid specific context
Encoded as a type II transmembrane trimer and shed by ADAM17, the product of Tnf (mouse ortholog of human TNF) engages TNFRSF1A and TNFRSF1B to nucleate complex I, in which TRADD, TRAF2, cIAP1/2, LUBAC, and RIPK1 ubiquitin chains recruit TAK1 and IKK for canonical NF-kB and JNK output, while RIPK1 deubiquitination diverts signaling into caspase-8 apoptosis or, when FADD or caspase-8 activity is limiting, RIPK3 and MLKL necroptosis. Allele choice tracks the question precisely: conditional null alleles test cell-intrinsic cytokine sources, the AU-rich element deletion knockin stabilizes transcript and reproduces Crohn-like ileitis and erosive arthritis, uncleavable membrane-only knockins separate juxtacrine from systemic activity, and full humanization is mandatory because infliximab and adalimumab do not recognize mouse TNF. Targeting must avoid disrupting the tightly linked Lta and Ltb loci, whose lymphotoxin products independently sustain lymphoid architecture and can confound readouts of germinal center or Peyer patch phenotypes.
A floxed Tnf allele paired with Cre gives spatial control that whole body knockouts cannot offer. People use it to separate developmental roles from adult homeostasis, to model somatic mutations, and to match disease that begins in one organ. For myeloid work, plan Cre specificity, reporter crosses, and baseline phenotyping before you scale.
Catalog options
Conditional knockout focuses the experiment on myeloid while the rest of the animal keeps a wild type allele. That pattern mirrors somatic mutation in patients and avoids systemic compensation that can erase subtle phenotypes. It is often preferred when a germline null is lethal, weak, or confounded by developmental rescue.
| Model | Type | Category | Availability | Catalog # | |
|---|---|---|---|---|---|
| Tnf-Flox | Conditional Knockout | KO/CKO mice | live | CKO 226490 | Inquire |
Designing a Tnf Conditional Knockout allele
Cell-source attribution is the point, and it is harder than usual because this cytokine acts at picomolar concentration, so deletion escapees can carry the phenotype. Design floxed sites entirely within the locus, keeping the neighboring lymphotoxin genes and the shared regulatory interval intact, and verify by sequencing that no enhancer used by Lta is removed. Pair drivers to the model: LysM-Cre for macrophage and neutrophil sources in endotoxemia, Cd4-Cre and Cd19-Cre for lymphocyte-derived cytokine in colitis and arthritis, Villin-Cre for intestinal epithelium, Cx3cr1-CreERT2 for microglia with tamoxifen timed to allow monocyte turnover. LysM-Cre deletes unevenly between neutrophils and tissue macrophages, so quantify recombination in sorted populations and measure cell-specific secretion ex vivo rather than serum alone. Endpoints include serum concentration after lipopolysaccharide, survival, joint histology with clinical scoring, and intestinal permeability.
Pricing and quotes
The Tnf Conditional Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Tnf allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with milestones for genotyping, QC, and dispatch.
FAQ
What Tnf Conditional Knockout mouse models are available?
When Tnf Conditional Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Tnf knockout embryonic lethal in mice?
It depends on background and allele design. Some Tnf germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for myeloid specific focused experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. Your note points to myeloid specific, so we start driver selection there.
Do you ship live Tnf animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Tnf?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.