What Tnf Conditional Knockout mouse models are available?

When Tnf Conditional Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.

Is Tnf knockout embryonic lethal in mice?

It depends on background and allele design. Some Tnf germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.

Which Cre driver is best for experiments?

Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.

Do you ship live Tnf animals?

When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.

How do I request a quote for Tnf?

Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.

●12 Models Available

READY TO SHIP!

LIVE · SPERM · EMBRYO · DEVELOPING

Tnf Conditional Knockout, Knockin, Knockout mouse models

Browse 12 Tnf mouse models including conditional knockout, knockin, knockout variants — in stock and ready to ship from ingenious targeting laboratory. Available as Tnf conditional knockout mouse, Tnf knockin mouse, Tnf knockout mouse, Tnf xenograft-applicable mouse. Request a quote within 24 hours.

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Why model Tnf

Encoded as a type II transmembrane trimer and shed by ADAM17, the product of Tnf (mouse ortholog of human TNF) engages TNFRSF1A and TNFRSF1B to nucleate complex I, in which TRADD, TRAF2, cIAP1/2, LUBAC, and RIPK1 ubiquitin chains recruit TAK1 and IKK for canonical NF-kB and JNK output, while RIPK1 deubiquitination diverts signaling into caspase-8 apoptosis or, when FADD or caspase-8 activity is limiting, RIPK3 and MLKL necroptosis. Allele choice tracks the question precisely: conditional null alleles test cell-intrinsic cytokine sources, the AU-rich element deletion knockin stabilizes transcript and reproduces Crohn-like ileitis and erosive arthritis, uncleavable membrane-only knockins separate juxtacrine from systemic activity, and full humanization is mandatory because infliximab and adalimumab do not recognize mouse TNF. Targeting must avoid disrupting the tightly linked Lta and Ltb loci, whose lymphotoxin products independently sustain lymphoid architecture and can confound readouts of germinal center or Peyer patch phenotypes.

What Tnf mouse models are available?

ingenious targeting laboratory offers 12 distinct Tnf (TNF) catalog mouse models, featuring conditional knockout, standard knockout, xenograft applicable lines, and knockin. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.

Model availability, allele class options, and quality control standards
Model Availability	Allele Class Options	Quality Control Standards
12 Ready Catalog Lines	Conditional Knockout (cKO)	Documented Germline Transmission
Custom Model Generation	Constitutive Knockout (KO)	Strict United States QC Guidelines
Advanced Modifications	Humanized, Knockin, Transgenic	Xenograft Applicable Strains

Quality Control: Documented Germline Transmission
Quality Control: Strict United States QC Guidelines
Quality Control: Xenograft Applicable Strains

Tnf model types

PathAvailability

Knockout
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Conditional knockout
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Knockin
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Humanized
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Transgenic / overexpression
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Prefer the order form? Order. Need an allele that is not listed? Request a quote.

Tnf allele types

Knockout, conditional, knockin, humanized, and related paths for Tnf (human TNF). Catalog lines ship when inventory exists. Everything else is a generation quote.

Knockout

Conventional / global / constitutive KO

Conditional KO

Inducible conditional KO

Tissue specific KO

Specify tissue or Cre driver on quote

Knockout first

Convertible floxed allele pathway

Double / compound KO

Multi allele / compound knockout project

Large scale / BAC deletion

BAC scale deletion or targeting

Knockin

Point mutation KI

cDNA KI

Reporter KI

Tag KI

Conditional KI

LSL or conditional expression knockin

Gene replacement KI

Humanized

Full gene replacement

Partial / domain humanized

Domain or partial humanization scope

Immune checkpoint humanized

Checkpoint IO humanization when gene is a checkpoint target

Single humanized

Double / multi humanized

Multi humanized / combination IO project

Targeted Transgenic

Safe harbor

Overexpression transgenic

BAC transgenic

BAC transgenic or large fragment insert

Cre / recombinase

Constitutive Cre driver

Inducible Cre

Tamoxifen or dox inducible Cre

Dual recombinase

Dual recombinase breeding scheme

Flp driver

Flp or FRT derivative allele pairing

Reporter

Gene specific reporter knockin

Standalone reporter catalog

Catalog reporter lines not tied to a single gene allele

Species / background

Rat KO / KI / transgenic

Specify rat on quote

Rabbit models

Specify rabbit on quote

C57BL/6

Background substrain on quote

BALB/c

129, NOD, NSG, FVB, CD1, DBA/2

Background strain on quote

Available Tnf catalog models

12 Tnf lines with QC documentation and technical support. Tnf floxed mice use loxP flanked alleles for Cre dependent tissue specific knockout.

Tnf-Flox
CKO
CKO
These mice carry loxP sites flanking exon 2-4 of Tnf gene. When crossed with a Cre recombinase-expressing strain, this strain is useful in eliminating tissue-specific conditional expression of Tnf gene.
LiveCKO 226490
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Tnf-EGFP
KI
Other
The Kozak-EGFP-WPRE-pA expression cassette was knocked into the Tnf gene start codon site.
In DevelopmentKI 263597
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Tnf-IRES-Luc
KI
Fluorescent Model
A IRES-Luciferase expression cassette was knocked into the Tnf gene stop codon site via CRISPR/Cas mediated recombination.
Embryo CryopreservedKI 200003
Order
Tnf-IRES-secNlucP
KI
Fluorescent Model
IRES-secNlucP expression cassette was knocked into the Tnf gene stop codon site.
Sperm CryopreservedKI 200274
Order
Tnf-HiBiT
KI
Other Toolbox (non-Cre, non-Fluorescent)
A HiBiT expression cassette was knocked into the Tnf gene stop codon site via CRISPR/Cas mediated recombination.
Embryo CryopreservedKI 190123
Order
Tnf-IRES-NanoLuc
KI
Fluorescent Model
A IRES-NanoLuc expression cassette was knocked into the Tnf gene stop codon site.
Embryo CryopreservedKI 190122
Order
Tnf-IRES-EGFP
KI
Other
The IRES-EGFP expression cassette was knocked into the Tnf gene stop codon site.
In DevelopmentKI 263599
Order
Tnf-2A-DTR-2A-EGFP
KI
Fluorescent Model, Other Toolbox (non-Cre, non-Fluorescent)
The P2A-DTR-T2A-EGFP expression cassette was knocked into the Tnf gene stop codon site.
Sperm CryopreservedKI 242160
Order
Tnf-KO(2)
KO
KO
The 3’UTR ARE of Tnf gene was deleted to generate Tnf knockout mice.
Sperm CryopreservedKO 230390
Order
Tnf-KO(3)
KO
KO, Disease Model
The exon 2-4 of Tnf gene was deleted to generate Tnf knockout mouse.
Sperm CryopreservedKO 232670
Order
Tnf-KO
KO
KO, Disease Model
Exon2-3 of Tnf gene were targeted to generate the Tnf knockout mice.
LiveKO 00100
Order
Tnf-KO/hTNFa-Tg
XA
Target-Humanized
The Tnf-KO (KO 00100) was crossed with hTNFa-Tg(TG 200004) to generate Tnf-KO/hTNFa-Tg mice.
In DevelopmentXA 263484
Order

Selected publications from ingenious targeting laboratory

Peer reviewed work involving Tnf (TNF) from ingenious supported projects.

CYLD Proteolysis Protects Macrophages from TNF-Mediated Auto-necroptosis Induced by LPS and Licensed by Type I IFNLegarda D, Justus SJ, Ang RL, Rikhi N, Li W, Moran TM, Zhang J, Mizoguchi E, Zelic M, Kelliher MA, Blander JM, Ting AT. (2016). Cell Rep 15(11): 2449-61

View all ingenious publications

Looking for a Tnf mouse model?

We have 12 Tnf models in stock — including Conditional Knockout, Knockin, Knockout types. In stock and ready to ship this week. All models come with full QC documentation, health certificates, and dedicated technical support.

Best pricing in the industry. Get a quote in 24 hours. Our team of PhD scientists is available to help you select the right model for your research.

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Models Available

Model Types

24 hrs

Quote Turnaround

Included

QC Documentation

About Tnf Mouse Model Types

A Tnf knockout mouse has the Tnf gene permanently inactivated, enabling loss of function studies. A Tnf conditional knockout (floxed) mouse carries loxP sites flanking a critical exon of Tnf, allowing Cre recombinase dependent deletion in specific tissues or at specific timepoints. Tnf knockin models carry a precisely inserted sequence at the Tnf locus, useful for reporter, tag, or humanization studies.

Knockout Mouse Models Conditional Knockout Mouse Models Knockin Mouse Models Mouse Model Generation Services

Modifications available

Jump to catalog backed pages for Tnf organized by modification type. Each URL is indexable and matches common search patterns.

Tnf Conditional Knockout Tnf Knockin Tnf Knockout Tnf Xenograft-Applicable Tnf cDNA Knockin Tnf Epitope Tag Knockin Tnf Humanized Tnf Inducible Knockout Tnf Reporter

Tissue-specific options

Catalog backed tissue and driver restricted routes for Tnf.

myeloid Tnf conditional knockout

Related Gene Targets

Tnfaip2 Tnfaip3 Tnfaip6 Tnfaip8 Tnfaip8l1 Tnfaip8l2 Tnfrsf10b Tnfrsf11a Tnfrsf11b Tnfrsf12a

Breed this line with ingenious targeting laboratory

Send the Tnf line to a U.S. barrier facility for colony maintenance, cohort production, and complex breeding schemes through mouse breeding services.

Mouse Models

Two paths. One scientific team.

Search 14,774+ study ready strains, or commission the exact knockout, knockin, or humanized model your program requires.

Live colonies

Tnf models ready to ship

Get a confirmed quote in 24 hours. All Tnf models ship with full QC documentation, health certificates, and lifetime technical support.

TnfStudy ready strains in catalog

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Model generation

Need a knockout, knockin, or humanized line?

Specialized conventional and conditional knockouts
Knockins including point mutations, cDNA insertions, and epitope tags
Humanized models, including partial and full gene replacements
Targeted transgenesis in safe harbor loci
Inducible/reversible alleles using advanced recombinase techniques

The same U.S. scientific team designs and delivers your allele when it is not on the shelf. 2,800+ custom models generated since 1998. 100% germline transmission guarantee.

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