Tnf Knockin mouse models
Tnf drives acute inflammation and underpins approved biologics for autoimmune disease. Global and tissue restricted mouse alleles clarified where neutralization helps versus impairs host defense. Conditional alleles help model cytokine storms and chronic ileitis with more anatomical precision. These lines still anchor many immunology screens.
An engineered Tnf allele can introduce a human coding region, a point change, or a fluorescent reporter without random transgene integration noise.
Catalog table
Knockin and humanized formats preserve regulatory context at the endogenous locus. For your target tissue focused programs, that matters when expression timing, splice isoforms, or allele dosage drive biology. Random integration transgenics can still help, but targeted alleles usually give cleaner pharmacology readouts.
Knockin and humanized formats preserve regulatory context at the endogenous locus. For liver focused programs, that matters when expression timing, splice isoforms, or allele dosage drive biology. Random integration transgenics can still help, but targeted alleles usually give cleaner pharmacology readouts.
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Tnf-2A-DTR-2A-EGFP | Knockin | fluorescent mouse, otherToolbox of Mice(Non-Cre, fluorescent) | sperm cryopreservation | KI 242160 | Inquire |
| Tnf-IRES-secNlucP | Knockin | fluorescent mouse | sperm cryopreservation | KI 200274 | Inquire |
| Tnf-IRES-NanoLuc | Knockin | fluorescent mouse | embryo cryopreservation | KI 190122 | Inquire |
| Tnf-IRES-Luc | Knockin | fluorescent mouse | embryo cryopreservation | KI 200003 | Inquire |
| Tnf-HiBiT | Knockin | otherToolbox of Mice(Non-Cre, fluorescent) | embryo cryopreservation | KI 190123 | Inquire |
Why this approach
Knockin and humanized formats preserve regulatory context at the endogenous locus. For your target tissue focused programs, that matters when expression timing, splice isoforms, or allele dosage drive biology. Random integration transgenics can still help, but targeted alleles usually give cleaner pharmacology readouts.
Knockin and humanized formats preserve regulatory context at the endogenous locus. For liver focused programs, that matters when expression timing, splice isoforms, or allele dosage drive biology. Random integration transgenics can still help, but targeted alleles usually give cleaner pharmacology readouts.
Build timeline and pricing
Typical custom projects target study ready cohorts near twenty six weeks from contract start when breeding is direct. Quotes return in about twenty four hours with milestones, pricing, and options for cryo or live dispatch.
Open the request quote formFAQ
How long does a Tnf knockin project take?
Most custom knockin projects run near twenty six weeks from contract activation to study ready animals when breeding is straightforward. Complex humanization or multi allele stacks can add time. We return detailed quotes within about twenty four hours so you can align cohort start dates with grant or IND milestones.
Is Tnf knockout embryonic lethal in mice?
Lethality depends on genetic background and exact allele design. Some Tnf germline knockouts are viable, others require conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null approaches before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak patterns. We map your organ and cell type to a short list of proven Cre lines, then discuss reporter crosses and controls. We prioritize drivers with strong community validation for your tissue.
Do you ship live Tnf animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a custom project with cryo or live dispatch options depending on cohort timing and geography.
How do I request a quote for Tnf?
Use the catalog inquire buttons or the request quote form with your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.