Tnf Knockout mouse models
Encoded as a type II transmembrane trimer and shed by ADAM17, the product of Tnf (mouse ortholog of human TNF) engages TNFRSF1A and TNFRSF1B to nucleate complex I, in which TRADD, TRAF2, cIAP1/2, LUBAC, and RIPK1 ubiquitin chains recruit TAK1 and IKK for canonical NF-kB and JNK output, while RIPK1 deubiquitination diverts signaling into caspase-8 apoptosis or, when FADD or caspase-8 activity is limiting, RIPK3 and MLKL necroptosis. Allele choice tracks the question precisely: conditional null alleles test cell-intrinsic cytokine sources, the AU-rich element deletion knockin stabilizes transcript and reproduces Crohn-like ileitis and erosive arthritis, uncleavable membrane-only knockins separate juxtacrine from systemic activity, and full humanization is mandatory because infliximab and adalimumab do not recognize mouse TNF. Targeting must avoid disrupting the tightly linked Lta and Ltb loci, whose lymphotoxin products independently sustain lymphoid architecture and can confound readouts of germinal center or Peyer patch phenotypes.
Tnf null animals are straightforward to genotype and phenotype when survival is acceptable. They remain a standard background for pharmacology, biomarker, and rescue studies.
What Tnf Knockout mouse models are available?
ingenious targeting laboratory offers 3 distinct Tnf knockout catalog mouse models. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Designing a Tnf Knockout allele
A complete null is right when the question is whether the cytokine is required at all, and wrong when the question is which form or which source matters, because it removes membrane and soluble activity together. Homozygotes are viable but show impaired containment of Listeria and mycobacteria with disorganized granulomas, blunted follicular dendritic cell networks, and resistance to lipopolysaccharide-induced shock and to collagen-induced arthritis, which makes the allele both an infection risk model and a negative control for anti-cytokine efficacy. Two design cautions apply. Because the locus sits beside Lta and Ltb, retained selection cassettes and large deletions in older lines can perturb lymphotoxin function and mimic the same lymphoid architecture defects, so use a cassette-free allele and genotype the neighbors. Backcross fully, since background dominates infection outcomes. Read bacterial burden, granuloma histology, splenic and Peyer patch organization, and survival after endotoxin.
A conventional knockout answers whether the gene is required broadly. When your target tissue is the organ of interest, a global null can still be informative if viability is acceptable and you want the simplest genotype. If the null is harsh, a floxed allele with a regional Cre is the safer long term platform.
Pricing and quotes
The Tnf Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Tnf allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Tnf Knockout mouse models are available?
When Tnf Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Tnf knockout embryonic lethal in mice?
It depends on background and allele design. Some Tnf germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Tnf animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Tnf?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.