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Tnf Knockin mouse models
Encoded as a type II transmembrane trimer and shed by ADAM17, the product of Tnf (mouse ortholog of human TNF) engages TNFRSF1A and TNFRSF1B to nucleate complex I, in which TRADD, TRAF2, cIAP1/2, LUBAC, and RIPK1 ubiquitin chains recruit TAK1 and IKK for canonical NF-kB and JNK output, while RIPK1 deubiquitination diverts signaling into caspase-8 apoptosis or, when FADD or caspase-8 activity is limiting, RIPK3 and MLKL necroptosis. Allele choice tracks the question precisely: conditional null alleles test cell-intrinsic cytokine sources, the AU-rich element deletion knockin stabilizes transcript and reproduces Crohn-like ileitis and erosive arthritis, uncleavable membrane-only knockins separate juxtacrine from systemic activity, and full humanization is mandatory because infliximab and adalimumab do not recognize mouse TNF. Targeting must avoid disrupting the tightly linked Lta and Ltb loci, whose lymphotoxin products independently sustain lymphoid architecture and can confound readouts of germinal center or Peyer patch phenotypes.
An engineered Tnf allele can introduce a human coding region, a point change, or a fluorescent reporter without random transgene integration noise.
What Tnf Knockin mouse models are available?
ingenious targeting laboratory offers 7 distinct Tnf knockin catalog mouse models. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Tnf-EGFP | KI | Other | DEVELOPING | KI 263597 | Inquire |
| The Kozak-EGFP-WPRE-pA expression cassette was knocked into the Tnf gene start codon site. | |||||
| Tnf-IRES-Luc | KI | Fluorescent Model | EMBRYO | KI 200003 | Inquire |
| A IRES-Luciferase expression cassette was knocked into the Tnf gene stop codon site via CRISPR/Cas mediated recombination. | |||||
| Tnf-IRES-secNlucP | KI | Fluorescent Model | SPERM | KI 200274 | Inquire |
| IRES-secNlucP expression cassette was knocked into the Tnf gene stop codon site. | |||||
| Tnf-HiBiT | KI | Other Toolbox (non-Cre, non-Fluorescent) | EMBRYO | KI 190123 | Inquire |
| A HiBiT expression cassette was knocked into the Tnf gene stop codon site via CRISPR/Cas mediated recombination. | |||||
| Tnf-IRES-NanoLuc | KI | Fluorescent Model | EMBRYO | KI 190122 | Inquire |
| A IRES-NanoLuc expression cassette was knocked into the Tnf gene stop codon site. | |||||
| Tnf-IRES-EGFP | KI | Other | DEVELOPING | KI 263599 | Inquire |
| The IRES-EGFP expression cassette was knocked into the Tnf gene stop codon site. | |||||
| Tnf-2A-DTR-2A-EGFP | KI | Fluorescent Model, Other Toolbox (non-Cre, non-Fluorescent) | SPERM | KI 242160 | Inquire |
| The P2A-DTR-T2A-EGFP expression cassette was knocked into the Tnf gene stop codon site. | |||||
Why this approach
Knockin and humanized formats keep regulatory context at the endogenous locus. For your target tissue focused programs, that matters when expression timing, splice isoforms, or allele dosage drive biology. Random integration transgenics can still help, but targeted alleles usually give cleaner pharmacology readouts.
Pricing and quotes
The Tnf Knockin lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Tnf allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Tnf Knockin mouse models are available?
When Tnf Knockin lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Tnf knockout embryonic lethal in mice?
It depends on background and allele design. Some Tnf germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Tnf animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Tnf?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.
Breed this line with ingenious targeting laboratory
Send the Tnf Knockin line to a U.S. barrier facility for colony maintenance, cohort production, and complex breeding schemes through mouse breeding services.