Tsc2 Knockout mouse models
Tuberin, encoded by Tsc2 (mouse ortholog of human TSC2), supplies the catalytic GAP domain that hydrolyzes RHEB-GTP on lysosomal membranes, and its loss produces ligand-independent mTORC1 activity, sustained S6K1 and 4E-BP1 phosphorylation, and secondary IRS1 degradation that dampens upstream AKT. Patient alleles range from truncations to GAP-domain missense substitutions and to variants that merely destabilize the hamartin interaction, which is why a disease-specific knockin answers a different question than a conditional null: the former resolves catalytic versus structural failure and genotype-phenotype severity, the latter defines lineage requirements. Homozygous deletion is embryonic lethal by mid-gestation, and heterozygotes develop renal cystadenoma and hepatic hemangioma, echoing the Eker rat. No paralog substitutes for tuberin, since TSC1 and TBC1D7 are stoichiometric partners rather than backups, so pair floxed alleles with kidney, brain, or smooth muscle Cre drivers and read out mTORC1 flux, lesion burden, and rapalog or ATP-competitive inhibitor response.
A conventional Tsc2 knockout removes gene function in all cells that inherit the allele. It is a proven first pass for target validation when redundancy is low.
What Tsc2 Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct Tsc2 knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Tsc2-KO | Knockout | KO/CKO mice | live | KO 251011 | Inquire |
Why this approach
A conventional knockout answers whether the gene is required broadly. When your target tissue is the organ of interest, a global null can still be informative if viability is acceptable and you want the simplest genotype. If the null is harsh, a floxed allele with a regional Cre is the safer long term platform.
Pricing and quotes
The Tsc2 Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Tsc2 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Tsc2 Knockout mouse models are available?
When Tsc2 Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Tsc2 knockout embryonic lethal in mice?
It depends on background and allele design. Some Tsc2 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Tsc2 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Tsc2?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.