U2af1 Knockout mouse models
Recognition of the 3 prime splice site depends on U2af1, the mouse ortholog of human U2AF1, the small subunit of the U2 auxiliary factor heterodimer, which uses two CCCH zinc fingers to contact the invariant AG dinucleotide and flanking nucleotides while its U2AF homology motif binds U2AF2 and licenses U2 snRNP loading at the branch point. Myeloid disease mutations are heterozygous and mutually exclusive with SF3B1 and SRSF2 lesions, with S34F and S34Y altering preference at the position three nucleotides upstream of the AG and Q157P or Q157R altering the position immediately downstream, producing distinct cassette exon and intron retention programs rather than global splicing failure; identical substitutions recur in lung adenocarcinoma. Because complete loss is not tolerated, the correct design is a conditional or doxycycline-inducible heterozygous hotspot knockin at the endogenous locus, induced with Mx1-cre or Vav1-cre to capture dysplastic hematopoiesis. Match allele to endpoint, whether isoform mapping, competitive repopulation, or spliceosome modulator sensitivity.
Global U2af1 deletion answers broad mechanism questions quickly. If timing or site matters, conditional alleles are a natural next step after the null is characterized.
What U2af1 Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct U2af1 knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| U2af1-KO | Knockout | KO/CKO mice | sperm cryopreservation | KO 205051 | Inquire |
Why this approach
A conventional knockout answers whether the gene is required broadly. When your target tissue is the organ of interest, a global null can still be informative if viability is acceptable and you want the simplest genotype. If the null is harsh, a floxed allele with a regional Cre is the safer long term platform.
Pricing and quotes
The U2af1 Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a U2af1 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What U2af1 Knockout mouse models are available?
When U2af1 Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is U2af1 knockout embryonic lethal in mice?
It depends on background and allele design. Some U2af1 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live U2af1 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for U2af1?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.