Conventional / global / constitutive KO
●2 Models Available
U2af1 Conditional Knockout, Knockout mouse models
Browse 2 U2af1 mouse models including conditional knockout, knockout variants from ingenious targeting laboratory. Available as U2af1 conditional knockout mouse, U2af1 knockout mouse. Contact us for availability and fast turnaround.
Why model U2af1
Recognition of the 3 prime splice site depends on U2af1, the mouse ortholog of human U2AF1, the small subunit of the U2 auxiliary factor heterodimer, which uses two CCCH zinc fingers to contact the invariant AG dinucleotide and flanking nucleotides while its U2AF homology motif binds U2AF2 and licenses U2 snRNP loading at the branch point. Myeloid disease mutations are heterozygous and mutually exclusive with SF3B1 and SRSF2 lesions, with S34F and S34Y altering preference at the position three nucleotides upstream of the AG and Q157P or Q157R altering the position immediately downstream, producing distinct cassette exon and intron retention programs rather than global splicing failure; identical substitutions recur in lung adenocarcinoma. Because complete loss is not tolerated, the correct design is a conditional or doxycycline-inducible heterozygous hotspot knockin at the endogenous locus, induced with Mx1-cre or Vav1-cre to capture dysplastic hematopoiesis. Match allele to endpoint, whether isoform mapping, competitive repopulation, or spliceosome modulator sensitivity.
What U2af1 mouse models are available?
ingenious targeting laboratory offers 2 distinct U2af1 (U2AF1) catalog mouse models, featuring conditional knockout and standard knockout. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
| Model Availability | Allele Class Options |
|---|---|
| 2 Ready Catalog Lines | Conditional Knockout (cKO) |
| Custom Model Generation | Constitutive Knockout (KO) |
| Advanced Modifications | Humanized, Knockin, Transgenic |
- Quality Control
- Documented Germline Transmission
- Quality Control
- Strict United States QC Guidelines
- Quality Control
- Cryo or Live Dispatch Options
U2af1 model types
PathAvailability
Knockout
Conditional knockout
Knockin
Humanized
Transgenic / overexpression
Prefer the order form? Order. Need an allele that is not listed? Request a quote.
U2af1 allele types
Knockout, conditional, knockin, humanized, and related paths for U2af1 (human U2AF1). Catalog lines ship when inventory exists. Everything else is a generation quote.
Knockout
Conditional KO (floxed / loxP)
Inducible conditional KO (CreER / tet)
Tissue specific KO
Specify tissue or Cre driver on quote
Knockout first (tm1a / IKMC)
Convertible floxed allele pathway
Double / compound KO
Multi allele / compound knockout project
Large scale / BAC deletion
BAC scale deletion or targeting
Knockin
Point mutation KI
cDNA KI
Reporter KI (GFP, YFP, RFP, mCherry, tdTomato, lacZ, luciferase)
Tag KI (FLAG, HA, Myc, V5)
Conditional KI (e.g. Rosa26 LSL)
LSL or conditional expression knockin
Gene replacement KI
Humanized
Full gene replacement
Partial / domain humanized
Domain or partial humanization scope
Immune checkpoint humanized
Checkpoint IO humanization when gene is a checkpoint target
Single humanized
Double / multi humanized
Multi humanized / combination IO project
Transgenic
Random integration transgenic
Safe harbor (ROSA26, HPRT, H11, Col1a1)
Overexpression transgenic
BAC transgenic
BAC transgenic or large fragment insert
Cre / recombinase
Constitutive Cre driver
Inducible Cre (CreER / tet)
Tamoxifen or dox inducible Cre
Dual recombinase (Cre+Dre / Cre+Flp)
Dual recombinase breeding scheme
Flp driver
Flp or FRT derivative allele pairing
Reporter
Reporter knockin (gene specific)
Standalone reporter catalog (lineage tracing, constitutive, dual reporter)
Catalog reporter lines not tied to a single gene allele
Species / background
Rat KO / KI / transgenic
Specify rat on quote
Rabbit models
Specify rabbit on quote
C57BL/6 (B6J / B6N)
Background substrain on quote
BALB/c
129, NOD, NSG, FVB, CD1, DBA/2
Background strain on quote
Available U2af1 catalog models
2 U2af1 lines with QC documentation and technical support. U2af1 floxed mice use loxP flanked alleles for Cre dependent tissue specific knockout.
Selected publications from ingenious targeting laboratory
Peer reviewed work involving U2af1 (U2AF1) from ITL supported projects.
- U2AF1 is a haplo-essential gene required for cancer cell survivalWadugu BA, Heard A, Srivatsan SN, Alberti MO, Ndonwi M, Grieb S, Bradley J, Shao J, Ahmed T, Shirai CL, Khanna A, Fei DL, Miller CA, Graubert TA, Walter MJ. (2021). J Clin Invest 131(21): e141401
Looking for a U2af1 mouse model?
We have 2 U2af1 models available — including Conditional Knockout, Knockout types. Contact us today for current availability and our fastest turnaround options. All models come with full QC documentation and technical support.
Best pricing in the industry. Get a quote in 24 hours. Our team of PhD scientists is available to help you select the right model for your research.
Order catalog model2
Models Available
2
Model Types
24 hrs
Quote Turnaround
Included
QC Documentation
About U2af1 Mouse Model Types
A U2af1 knockout mouse has the U2af1 gene permanently inactivated, enabling loss of function studies. A U2af1 conditional knockout (floxed) mouse carries loxP sites flanking a critical exon of U2af1, allowing Cre recombinase dependent deletion in specific tissues or at specific timepoints.
Modifications available
Jump to catalog backed pages for U2af1 organized by modification type. Each URL is indexable and matches common search patterns.