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Vapb Conditional Knockout mouse models
Endoplasmic reticulum membrane contact sites are organized in part by Vapb, the mouse ortholog of human VAPB, encoding a tail-anchored ER protein whose cytosolic major sperm protein domain binds FFAT motifs in lipid transfer and tethering partners such as OSBP, ORP family proteins, STARD3, and PTPIP51, coupling the ER to mitochondria, endosomes, and the plasma membrane for sterol, phosphatidylserine, and calcium exchange. The dominant P56S mutation misfolds that domain, forming insoluble ER inclusions that sequester wild type VAPB and VAPA, causing ALS8 and late-onset spinal muscular atrophy, while a cleaved fragment can be secreted and act on Eph receptors. Model design should separate P56S knockin at the endogenous locus, which captures the dominant aggregation mechanism, from conditional null, which tests contact site function; note that Vapb nulls are viable with only mild late deficits. Genuine redundancy with Vapa argues for compound alleles when lipid transfer is the endpoint.
A floxed Vapb allele paired with Cre gives spatial control that whole body knockouts cannot offer. People use it to separate developmental roles from adult homeostasis, to model somatic mutations, and to match disease that begins in one organ.
What Vapb Conditional Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct Vapb conditional knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Vapb-Flox | CKO | CKO | DEVELOPING | CKO 262927 | Inquire |
| These mice carry loxP sites flanking exon3 of Vapb gene. When crossed with a Cre recombinase-expressing strain, this strain is useful in eliminating tissue-specific conditional expression of Vapb gene. | |||||
Why this approach
Conditional knockout focuses the experiment on your target tissue while the rest of the animal keeps a wild type allele. That pattern mirrors somatic mutation in patients and avoids systemic compensation that can erase subtle phenotypes. It is often preferred when a germline null is lethal, weak, or confounded by developmental rescue.
Pricing and quotes
The Vapb Conditional Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Vapb allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Vapb Conditional Knockout mouse models are available?
When Vapb Conditional Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Vapb knockout embryonic lethal in mice?
It depends on background and allele design. Some Vapb germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Vapb animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Vapb?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.
Breed this line with ingenious targeting laboratory
Send the Vapb Conditional Knockout line to a U.S. barrier facility for colony maintenance, cohort production, and complex breeding schemes through mouse breeding services.