Vegfa Conditional Knockout mouse — endothelial specific context
Vegfa, the mouse ortholog of human VEGFA, encodes the dimeric heparin-binding growth factor spliced into 120, 164, and 188 residue isoforms that differ in exon 6 and 7 matrix retention and signal through KDR (VEGFR2) autophosphorylation to PLCG1, PKC, and ERK, with FLT1 acting as ligand sink and neuropilins as coreceptors during DLL4-Notch-governed tip cell selection. Unusually, this locus is haploinsufficient: even heterozygous deletion is lethal around E11 from failed endothelial assembly, so conditional floxed alleles paired with tightly validated Cdh5, Sox2, or lineage-restricted Cre drivers are mandatory rather than optional, and isoform-restricted knockins expressing only the 120 or 188 form dissect gradient formation from bulk mitogenic dose. Humanization matters because bevacizumab and ranibizumab do not neutralize mouse VEGF-A. Vegfb and Pgf engage FLT1 alone and cannot restore KDR signaling, so map alleles onto oxygen-induced retinopathy, permeability, or tumor angiogenesis endpoints.
Conditional deletion of Vegfa limits the genetic change to the lineage you choose. That helps in oncology, immunology, and metabolic work where systemic loss would muddy the read. After you confirm Cre specificity, crossing to Vegfa floxed stock yields usable cohorts. For endothelial work, plan Cre specificity, reporter crosses, and baseline phenotyping before you scale.
Catalog options
Conditional knockout focuses the experiment on endothelial while the rest of the animal keeps a wild type allele. That pattern mirrors somatic mutation in patients and avoids systemic compensation that can erase subtle phenotypes. It is often preferred when a germline null is lethal, weak, or confounded by developmental rescue.
| Model | Type | Category | Availability | Catalog # | |
|---|---|---|---|---|---|
| Vegfa-Flox(2) | Conditional Knockout | KO/CKO mice | sperm cryopreservation | CKO 234739 | Inquire |
Pricing and quotes
The Vegfa Conditional Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Vegfa allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with milestones for genotyping, QC, and dispatch.
FAQ
What Vegfa Conditional Knockout mouse models are available?
When Vegfa Conditional Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Vegfa knockout embryonic lethal in mice?
It depends on background and allele design. Some Vegfa germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for endothelial specific focused experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. Your note points to endothelial specific, so we start driver selection there.
Do you ship live Vegfa animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Vegfa?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.