Vegfa Conditional Knockout mouse models
Vegfa, the mouse ortholog of human VEGFA, encodes the dimeric heparin-binding growth factor spliced into 120, 164, and 188 residue isoforms that differ in exon 6 and 7 matrix retention and signal through KDR (VEGFR2) autophosphorylation to PLCG1, PKC, and ERK, with FLT1 acting as ligand sink and neuropilins as coreceptors during DLL4-Notch-governed tip cell selection. Unusually, this locus is haploinsufficient: even heterozygous deletion is lethal around E11 from failed endothelial assembly, so conditional floxed alleles paired with tightly validated Cdh5, Sox2, or lineage-restricted Cre drivers are mandatory rather than optional, and isoform-restricted knockins expressing only the 120 or 188 form dissect gradient formation from bulk mitogenic dose. Humanization matters because bevacizumab and ranibizumab do not neutralize mouse VEGF-A. Vegfb and Pgf engage FLT1 alone and cannot restore KDR signaling, so map alleles onto oxygen-induced retinopathy, permeability, or tumor angiogenesis endpoints.
A floxed Vegfa allele paired with Cre gives spatial control that whole body knockouts cannot offer. People use it to separate developmental roles from adult homeostasis, to model somatic mutations, and to match disease that begins in one organ.
What Vegfa Conditional Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct Vegfa conditional knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Vegfa-Flox(2) | Conditional Knockout | KO/CKO mice | sperm cryopreservation | CKO 234739 | Inquire |
Why this approach
Conditional knockout focuses the experiment on your target tissue while the rest of the animal keeps a wild type allele. That pattern mirrors somatic mutation in patients and avoids systemic compensation that can erase subtle phenotypes. It is often preferred when a germline null is lethal, weak, or confounded by developmental rescue.
Pricing and quotes
The Vegfa Conditional Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Vegfa allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Vegfa Conditional Knockout mouse models are available?
When Vegfa Conditional Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Vegfa knockout embryonic lethal in mice?
It depends on background and allele design. Some Vegfa germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Vegfa animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Vegfa?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.