Vhl Xenograft-Applicable mouse models
Vhl encodes the mouse ortholog of human VHL, the substrate recognition subunit of a cullin 2 RING E3 ubiquitin ligase that assembles with elongin B, elongin C, CUL2, and RBX1 and binds hydroxylated prolines in the oxygen-dependent degradation domains of HIF1A and HIF2A after prolyl hydroxylase modification, thereby coupling oxygen and 2-oxoglutarate availability to hypoxia inducible factor destruction. Biallelic inactivation, usually 3p25 deletion plus a truncating or missense hit, initiates clear cell renal carcinoma and drives von Hippel-Lindau disease with hemangioblastoma, pheochromocytoma, and pancreatic neuroendocrine tumors, with clear genotype specificity since type 2C alleles retain HIF regulation while losing other functions. Germline nulls die in midgestation from placental vasculogenesis failure, so conditional nulls with Pax8-cre or Ksp-cadherin-cre are standard, generally combined with Pbrm1, Bap1, or Setd2 deletion because Vhl loss alone rarely produces carcinoma in mouse kidney. Choose nulls for HIF2A dependence and belzutifan response, missense knockins for genotype-phenotype dissection.
Vhl engineered hosts can be tuned for xenograft take rate and growth kinetics depending on tumor lineage.
What Vhl Xenograft-Applicable mouse models are available?
ingenious targeting laboratory offers 1 distinct Vhl xenograft-applicable catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Vhl-Flox/Bap1-Flox | Xenograft-Applicable | KO/CKO mice | live | XA 241192 | Inquire |
Why this approach
We help teams pick alleles that match the experimental question, the organ system, and future breeding plans. For your target tissue centric work, Cre specificity, flox placement, and baseline controls matter as much as the gene edit itself.
Pricing and quotes
The Vhl Xenograft-Applicable lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Vhl allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Vhl Xenograft-Applicable mouse models are available?
When Vhl Xenograft-Applicable lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Vhl knockout embryonic lethal in mice?
It depends on background and allele design. Some Vhl germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Vhl animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Vhl?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.