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Wnt3a Conditional Knockout mouse models
Widely supplied as the recombinant canonical ligand of choice, Wnt3a is the mouse ortholog of human WNT3A and stabilizes beta-catenin through Frizzled with LRP6 to maintain the primitive streak after initiation, sustain neuromesodermal progenitors, and drive paraxial mesoderm and somitogenesis, with an additional requirement in hippocampal development alongside LEF1. Nulls truncate the caudal axis with absent tailbud somites and die in late gestation, whereas the spontaneous vestigial tail allele is hypomorphic and survives, providing a natural allelic series that argues for dose-graded engineering rather than a single null. Conditional deletion isolates progenitor pool maintenance from later neural roles, hypomorphic knockins titrate signaling output against segment number, and humanization supports agonist and surrogate ligand testing. Wnt3 overlaps in axis programs and Wnt5a contributes to elongation, so pair conditional alleles with a validated posterior driver and a beta-catenin reporter to quantify actual pathway dose.
Tissue restricted knockout of Wnt3a keeps wild type function everywhere else. Breeding is often more robust, and the setup mirrors patient biology where mutations arise in a subset of cells.
What Wnt3a Conditional Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct Wnt3a conditional knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Wnt3a-Flox | CKO | CKO | DEVELOPING | CKO 264040 | Inquire |
| These mice carry loxP sites flanking exon2 of Wnt3a gene. When crossed with a Cre recombinase-expressing strain, this strain is useful in eliminating tissue-specific conditional expression of Wnt3a gene. | |||||
Why this approach
Conditional knockout focuses the experiment on your target tissue while the rest of the animal keeps a wild type allele. That pattern mirrors somatic mutation in patients and avoids systemic compensation that can erase subtle phenotypes. It is often preferred when a germline null is lethal, weak, or confounded by developmental rescue.
Pricing and quotes
The Wnt3a Conditional Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Wnt3a allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Wnt3a Conditional Knockout mouse models are available?
When Wnt3a Conditional Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Wnt3a knockout embryonic lethal in mice?
It depends on background and allele design. Some Wnt3a germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Wnt3a animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Wnt3a?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.
Breed this line with ingenious targeting laboratory
Send the Wnt3a Conditional Knockout line to a U.S. barrier facility for colony maintenance, cohort production, and complex breeding schemes through mouse breeding services.