Xiap Knockout mouse models
Xiap is the mouse ortholog of human XIAP and encodes an inhibitor of apoptosis protein with three BIR domains, a ubiquitin-associated region, and a C-terminal RING E3 ligase, in which BIR2 plus its preceding linker occludes the active sites of caspase-3 and caspase-7 while BIR3 binds and holds caspase-9 monomers inactive, and the RING ubiquitinates caspases and other substrates for degradation. SMAC (Diablo) released from mitochondria displaces these interactions through its IAP-binding motif, the principle exploited by SMAC mimetics. Independently of apoptosis, XIAP ubiquitinates RIPK2 to sustain NOD1 and NOD2 driven NF-kB signaling, which is why human loss of function causes X-linked lymphoproliferative syndrome type 2 with hemophagocytic lymphohistiocytosis and Crohn-like disease. Nulls are viable with compensatory Birc2 and Birc3 upregulation, so compound alleles or RING-dead and BIR-selective knockins give sharper answers, and allele choice should follow NOD2 responsiveness, inflammasome activation, or SMAC mimetic sensitivity.
Xiap null animals are straightforward to genotype and phenotype when survival is acceptable. They remain a standard background for pharmacology, biomarker, and rescue studies.
What Xiap Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct Xiap knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Xiap-KO | Knockout | KO/CKO mice | sperm cryopreservation | KO 190176 | Inquire |
Why this approach
A conventional knockout answers whether the gene is required broadly. When your target tissue is the organ of interest, a global null can still be informative if viability is acceptable and you want the simplest genotype. If the null is harsh, a floxed allele with a regional Cre is the safer long term platform.
Pricing and quotes
The Xiap Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Xiap allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Xiap Knockout mouse models are available?
When Xiap Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Xiap knockout embryonic lethal in mice?
It depends on background and allele design. Some Xiap germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Xiap animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Xiap?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.