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Zbtb16 Conditional Knockout mouse models
Zbtb16 is the mouse ortholog of human ZBTB16 (PLZF), a transcriptional repressor combining an N-terminal BTB or POZ dimerization domain with nine C-terminal C2H2 zinc fingers, which recruits NCOR1, SMRT, HDAC, and PRC1 components to target promoters and enforces spermatogonial stem cell self-renewal, innate effector programming of invariant natural killer T cells, and limb and axial skeletal patterning. In oncology it appears as the 5 prime partner of the t(11;17) ZBTB16-RARA fusion in a rare, all-trans retinoic acid resistant variant of acute promyelocytic leukemia, where the BTB domain drives constitutive oligomerization and corepressor recruitment that pharmacologic retinoid concentrations cannot dislodge. Germline nulls are viable, phenocopying the luxoid mutant with limb and vertebral defects and progressive loss of spermatogonial stem cells, so conditional nulls suit adult germline and immune endpoints, whereas inducible fusion knockins address differentiation therapy resistance. No close paralog substitutes for this BTB and zinc finger combination, so single-locus deletion is interpretable with a lineage-matched Cre.
A floxed Zbtb16 allele paired with Cre gives spatial control that whole body knockouts cannot offer. People use it to separate developmental roles from adult homeostasis, to model somatic mutations, and to match disease that begins in one organ.
What Zbtb16 Conditional Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct Zbtb16 (PLZF) conditional knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Zbtb16-Flox | CKO | CKO | LIVE | CKO 253300 | Inquire |
| These mice carry loxP sites flanking exon 3 of Zbtb16 gene. When crossed with a Cre recombinase-expressing strain, this strain is useful in eliminating tissue-specific conditional expression of Zbtb16 gene. | |||||
Why this approach
Conditional knockout focuses the experiment on your target tissue while the rest of the animal keeps a wild type allele. That pattern mirrors somatic mutation in patients and avoids systemic compensation that can erase subtle phenotypes. It is often preferred when a germline null is lethal, weak, or confounded by developmental rescue.
Pricing and quotes
The Zbtb16 Conditional Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Zbtb16 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Zbtb16 Conditional Knockout mouse models are available?
When Zbtb16 Conditional Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Zbtb16 knockout embryonic lethal in mice?
It depends on background and allele design. Some Zbtb16 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Zbtb16 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Zbtb16?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.
Breed this line with ingenious targeting laboratory
Send the Zbtb16 Conditional Knockout line to a U.S. barrier facility for colony maintenance, cohort production, and complex breeding schemes through mouse breeding services.