A knockout mouse is a mouse in which an existing gene has been inactivated, or “knocked out,” by deleting portions of the gene’s DNA sequence to make it nonfunctional, or by replacing the gene with an altered DNA sequence. ingenious began as one of the pioneering companies delivering knockout mice to researchers worldwide. From conventional deletions to conditional knockouts and large scale deletions using BACs, we have developed the technology and expertise to target even the most challenging genes. Working with ingenious targeting laboratory, our clients know they have chosen a company with longevity, backed by almost two decades of successful, published experience with knockout mice.
The target region is deleted or replaced with a reporter gene. The targeted gene is thus inactivated at all times, in all tissues.
The target region is flanked by loxP sites (“floxed”) to facilitate Cre recombinase-mediated excision, leading to tissue-specific gene inactivation.
Learn more about ingenious’ core knockout specialties:
- Targeted gene knockout mice with multiple options for strategy, including a CRISPR/Cas9 knockout.
- Delete as little as 200bp, or as much as 200kb using our BAC technology.
- Enhance the versatility of your knockout mice by combining reporter gene expression with your targeted knockout.
Work with ingenious
for your custom knockout mouse model and enjoy:
The model you design is the model we deliver.
Assurance of your mouse model, not just your money back.
Our proprietary technologies save time and money.
You will receive a quote within 24 hours, and one of our technical consultants will contact you to discuss your next mouse model.
“I am very happy with iTL’s mouse knockout services. It was pleasant to collaborate with my project manager. She was very thorough and communicative, an excellent combination. I will certainly use iTL again when needed, and I’m happy to share my positive experience with colleagues.”
-John Creemers, PhD