Skip to main content
Back to Glossary
Vectors, ES Cells & Delivery

Large-Fragment / BAC Targeting

Introducing extended genomic DNA segments—often spanning 100–300 kilobases—into the mouse genome using bacterial artificial chromosomes (BACs) to preserve native gene context and regulatory elements.

Overview

Large-fragment or BAC (bacterial artificial chromosome) targeting involves introducing extended genomic DNA segments—often spanning 100–300 kilobases—into the mouse genome to preserve native gene context and regulatory elements.

Frequently Asked Questions

When should BAC targeting be used?

BAC targeting is particularly valuable for humanized gene models, reporter lines, rescue constructs, and multigene loci where cDNA constructs cannot capture regulatory complexity.

Related Services

Related Terms

Targeting Vector (Homology Arms)

A synthetic DNA construct designed to introduce specific genetic modifications into a genome through homologous recombination or HDR. It contains sequences known as homology arms that align precisely with the genomic target locus.

Humanized Mouse Models

A genetically engineered mouse in which one or more human genes, immune system components, or biological pathways have been introduced to replicate aspects of human physiology, bridging the gap between basic research and clinical translation.

More in Vectors, ES Cells & Delivery

Targeting Vector (Homology Arms)Selection Markers (NeoR, PuroR)Blastocyst Injection / Chimera ProductionPronuclear Injection (Transgenics)Single-Copy Integration
Knockout (KO) Mouse ModelsView All TermsLox-STOP-Lox (LSL) Cassette

Need Help with Your Mouse Model Project?

Our scientific consultants can help you understand the best approach for your research goals.

Request a QuoteContact Us