Aldh1a1 Knockout mouse models
Aldh1a1, the mouse ortholog of human ALDH1A1, encodes the cytosolic NAD+-dependent retinaldehyde dehydrogenase RALDH1, which performs the irreversible oxidation of retinaldehyde to all-trans retinoic acid and thereby supplies ligand to nuclear RAR and RXR heterodimers in dorsal retina, liver, lung, and visceral adipose tissue. The same tetrameric active site detoxifies reactive lipid aldehydes such as 4-hydroxynonenal and metabolizes oxazaphosphorine chemotherapeutics, which is why high enzyme activity defines ALDEFLUOR-positive tumor populations and cyclophosphamide resistance. Since germline nulls are viable and fertile, constitutive deletion suits metabolic and adipose questions, whereas conditional floxed alleles isolate autonomous requirements in retina or tumor compartments, and catalytically dead point knockins separate enzymatic output from noncatalytic roles. Be aware that Aldh1a2, Aldh1a3, and Aldh8a1 generate retinoic acid in overlapping domains, so single-locus loss is often buffered. Humanized knockins are the correct substrate for isoform-selective inhibitor pharmacology, while compound Raldh genetics is required to collapse local retinoid signaling outright.
A conventional Aldh1a1 knockout removes gene function in all cells that inherit the allele. It is a proven first pass for target validation when redundancy is low.
What Aldh1a1 Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct Aldh1a1 knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Aldh1a1-KO | Knockout | KO/CKO mice | sperm cryopreservation | KO 250645 | Inquire |
Why this approach
A conventional knockout answers whether the gene is required broadly. When your target tissue is the organ of interest, a global null can still be informative if viability is acceptable and you want the simplest genotype. If the null is harsh, a floxed allele with a regional Cre is the safer long term platform.
Pricing and quotes
The Aldh1a1 Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Aldh1a1 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Aldh1a1 Knockout mouse models are available?
When Aldh1a1 Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Aldh1a1 knockout embryonic lethal in mice?
It depends on background and allele design. Some Aldh1a1 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Aldh1a1 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Aldh1a1?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.