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Aldh1a2 Knockout mouse models
Encoding RALDH2, Aldh1a2 is the mouse ortholog of human ALDH1A2 and the dominant source of embryonic all-trans retinoic acid, oxidizing retinaldehyde within paraxial and lateral plate mesoderm, epicardium, and meninges to establish the posterior-to-anterior retinoid gradient read by RAR and Hox targets. Loss of this single enzyme is not tolerated: germline nulls arrest around midgestation with unlooped heart tubes, absent forelimb buds, shortened trunks, and disrupted hindbrain segmentation, a lethality partially rescuable by maternal retinoic acid supplementation, so timed dietary rescue must be planned into any constitutive line. That embryonic requirement makes conditional floxed alleles the practical design for adult immunology, where dendritic cell and stromal RALDH2 drives gut homing and regulatory T cell induction. Paralogous Aldh1a1 and Aldh1a3 occupy largely nonoverlapping domains and do not rescue the early mesodermal deficit. Pair the flox with tissue-restricted Cre drivers such as Cd11c-Cre or Wt1-Cre when the endpoint is retinoid-dependent cell fate rather than gross morphogenesis.
Global Aldh1a2 deletion answers broad mechanism questions quickly. If timing or site matters, conditional alleles are a natural next step after the null is characterized.
What Aldh1a2 Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct Aldh1a2 (RALDH2) knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Aldh1a2-KO | KO | KO | LIVE | KO 260298 | Inquire |
| The exon 2-4 of Aldh1a2 gene was deleted to generate Aldh1a2 knockout mouse. | |||||
Why this approach
A conventional knockout answers whether the gene is required broadly. When your target tissue is the organ of interest, a global null can still be informative if viability is acceptable and you want the simplest genotype. If the null is harsh, a floxed allele with a regional Cre is the safer long term platform.
Pricing and quotes
The Aldh1a2 Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Aldh1a2 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Aldh1a2 Knockout mouse models are available?
When Aldh1a2 Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Aldh1a2 knockout embryonic lethal in mice?
It depends on background and allele design. Some Aldh1a2 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Aldh1a2 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Aldh1a2?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.
Breed this line with ingenious targeting laboratory
Send the Aldh1a2 Knockout line to a U.S. barrier facility for colony maintenance, cohort production, and complex breeding schemes through mouse breeding services.