Apc Knockin mouse models
Apc, the mouse ortholog of human APC, encodes the large scaffold that nucleates the beta-catenin destruction complex, using 15 amino acid and 20 amino acid repeats to bind beta-catenin and SAMP repeats to bind AXIN1 and AXIN2, thereby positioning CK1 alpha and GSK3 beta for the priming and processive phosphorylations that create the beta-TrCP degron. Independently, the armadillo repeat region and C-terminal EB1 and DLG binding sites control microtubule plus end dynamics and mitotic spindle fidelity, so truncating alleles produce both Wnt hyperactivation and chromosomal instability. Mutation position matters enormously: the mutation cluster region retains some repeats and yields intermediate signaling, which is why allelic series such as Min, 1638N and 1322T give different tumor multiplicities. Homozygous null is embryonic lethal, mandating floxed exon 14 or 15 designs with tissue-restricted Cre for intestinal, hepatic or mammary work. Match truncation position to the desired Wnt setpoint rather than assuming all Apc alleles are equivalent.
Knockin designs at Apc support head to head comparisons between wild type and modified alleles because regulatory DNA stays in place.
What Apc Knockin mouse models are available?
ingenious targeting laboratory offers 2 distinct Apc knockin catalog mouse models. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Why this approach
Knockin and humanized formats keep regulatory context at the endogenous locus. For your target tissue focused programs, that matters when expression timing, splice isoforms, or allele dosage drive biology. Random integration transgenics can still help, but targeted alleles usually give cleaner pharmacology readouts.
Pricing and quotes
The Apc Knockin lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Apc allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Apc Knockin mouse models are available?
When Apc Knockin lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Apc knockout embryonic lethal in mice?
It depends on background and allele design. Some Apc germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Apc animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Apc?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.