Arid1a Conditional Knockout mouse models
Arid1a encodes BAF250a, the mouse ortholog of human ARID1A and the largest accessory subunit of the canonical BAF chromatin remodeling complex, where its ARID domain contributes non-sequence-specific DNA contact and its C-terminal region docks onto the SMARCA4 ATPase to target enhancer nucleosome eviction. Tumor alleles are overwhelmingly nonsense or frameshift, collapsing accessibility at lineage and differentiation enhancers in ovarian clear cell carcinoma, endometrioid tumors, gastric adenocarcinoma, and hepatocellular carcinoma. Homozygous deletion causes gastrulation-stage embryonic lethality near E6.5, so useful models require conditional flox alleles with tissue-restricted Cre, frequently combined with Pten or Trp53 loss for penetrant tumors. The paralog Arid1b occupies the same BAF slot in mutually exclusive fashion and buffers single-locus loss, which is precisely why ARID1A mutant cells become selectively dependent on ARID1B, EZH2, and ATR. Choose heterozygous or mosaic loss for dosage questions and full conditional deletion when validating synthetic lethal targets.
Arid1a conditional knockout mice carry a floxed allele so you delete function only where Cre is active. The germline allele stays intact until you cross to a tissue specific Cre. Labs reach for this design when a global knockout is lethal, when they need adult onset loss, or when regional redundancy hides a whole body phenotype.
What Arid1a Conditional Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct Arid1a conditional knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Arid1a-Flox | Conditional Knockout | KO/CKO mice, disease model mice | live | CKO 200078 | Inquire |
Why this approach
Conditional knockout focuses the experiment on your target tissue while the rest of the animal keeps a wild type allele. That pattern mirrors somatic mutation in patients and avoids systemic compensation that can erase subtle phenotypes. It is often preferred when a germline null is lethal, weak, or confounded by developmental rescue.
Pricing and quotes
The Arid1a Conditional Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Arid1a allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Arid1a Conditional Knockout mouse models are available?
When Arid1a Conditional Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Arid1a knockout embryonic lethal in mice?
It depends on background and allele design. Some Arid1a germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Arid1a animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Arid1a?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.