Bmp3 Knockout mouse models
Functionally inverted relative to its osteogenic relatives, Bmp3, the mouse ortholog of human BMP3 and historically called osteogenin, encodes an abundant bone matrix ligand that antagonizes bone formation by signaling through ACVR2B with ALK4 to phosphorylate SMAD2 and SMAD3, thereby competing with SMAD1, SMAD5, and SMAD8 output from BMP2, BMP4, and BMP7 and restraining osteoprogenitor commitment. Deletion in mice is viable and produces increased trabecular bone volume, establishing the gene as a negative regulator whose loss is an anabolic gain, while transgenic overexpression yields spontaneous rib fractures and reduced bone mass. That directionality dictates allele choice: constitutive nulls address bone accrual and fracture healing, conditional floxed alleles separate osteocyte-derived from marrow stromal ligand, and overexpression knockins model the osteopenic extreme. Its closest paralog Gdf10, also named BMP3B, shares SMAD2 and SMAD3 bias and can obscure single-locus effects in skeletal readouts. Align allele class with microCT, dynamic histomorphometry, or callus bridging endpoints accordingly.
A conventional Bmp3 knockout removes gene function in all cells that inherit the allele. It is a proven first pass for target validation when redundancy is low.
What Bmp3 Knockout mouse models are available?
ingenious targeting laboratory offers 2 distinct Bmp3 knockout catalog mouse models. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Why this approach
A conventional knockout answers whether the gene is required broadly. When your target tissue is the organ of interest, a global null can still be informative if viability is acceptable and you want the simplest genotype. If the null is harsh, a floxed allele with a regional Cre is the safer long term platform.
Pricing and quotes
The Bmp3 Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Bmp3 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Bmp3 Knockout mouse models are available?
When Bmp3 Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Bmp3 knockout embryonic lethal in mice?
It depends on background and allele design. Some Bmp3 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Bmp3 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Bmp3?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.