Brca2 Conditional Knockout mouse — liver specific context
Encoding the RAD51 loading factor, Brca2 is the mouse ortholog of human BRCA2 and produces a very large protein whose eight BRC repeats bind RAD51 monomers, whose DNA binding domain with the DSS1 partner engages single stranded and double stranded junctions, and whose C-terminal TR2 site stabilizes assembled filaments, together enabling strand invasion during homologous recombination and protecting stalled replication forks from MRE11 mediated degradation. Because fork protection and HR are genetically separable, allele design matters: full nulls are embryonic lethal, whereas hypomorphic truncation alleles and conditional floxed exon 11 designs permit viable tumor-prone cohorts, typically combined with Trp53 deletion and a mammary or pancreatic Cre. Biallelic hypomorphic human alleles cause Fanconi anemia subtype D1, providing a distinct developmental readout. Reversion and fork protection restoring mutations drive PARP inhibitor resistance in vivo, so knockins of specific patient variants are the right tool for resistance mechanism work rather than a generic null.
Conditional deletion of Brca2 limits the genetic change to the lineage you choose. That helps in oncology, immunology, and metabolic work where systemic loss would muddy the read. After you confirm Cre specificity, crossing to Brca2 floxed stock yields usable cohorts. For liver work, plan Cre specificity, reporter crosses, and baseline phenotyping before you scale.
Catalog options
Conditional knockout focuses the experiment on liver while the rest of the animal keeps a wild type allele. That pattern mirrors somatic mutation in patients and avoids systemic compensation that can erase subtle phenotypes. It is often preferred when a germline null is lethal, weak, or confounded by developmental rescue.
| Model | Type | Category | Availability | Catalog # | |
|---|---|---|---|---|---|
| Brca2-Flox | Conditional Knockout | KO/CKO mice, disease model mice | sperm cryopreservation | CKO 200014 | Inquire |
Designing a Brca2 Conditional Knockout allele
Conditional deletion of Brca2 is dominated by one practical problem: recombined cells that lack p53 support are counter-selected, and tumors that eventually appear are frequently escapers that retained an unrecombined allele. Every cohort therefore needs allele-specific PCR on tumor DNA, ideally on laser-captured epithelium, to confirm that the lesion is genuinely null. Which exons you flank determines what question the allele can answer. Removing the BRC repeat exon abolishes RAD51 monomer loading and collapses homologous recombination outright. Deleting only the carboxy-terminal exon that carries the filament-stabilizing site leaves recombination broadly intact while stripping stalled fork protection, which is the cleaner allele for asking whether fork degradation alone drives chemosensitivity. For drivers, K14-Cre and beta-lactoglobulin-Cre give basal and luminal mammary targeting respectively, and Pdx1-Cre serves pancreatic work. Score nascent strand degradation by DNA fiber assay alongside tumor latency.
Pricing and quotes
The Brca2 Conditional Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Brca2 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with milestones for genotyping, QC, and dispatch.
FAQ
What Brca2 Conditional Knockout mouse models are available?
When Brca2 Conditional Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Brca2 knockout embryonic lethal in mice?
It depends on background and allele design. Some Brca2 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for liver specific focused experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. Your note points to liver specific, so we start driver selection there.
Do you ship live Brca2 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Brca2?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.