Cdk4 Knockout mouse models
Cdk4, mouse ortholog of human CDK4, encodes the D-cyclin dependent kinase that initiates RB phosphorylation at the restriction point, is directly inhibited by the INK4 proteins p16, p15, p18 and p19 through an allosteric interface that distorts the ATP site, and requires assembly with p21 or p27 plus CAK activation to function. The R24C substitution abolishes INK4 binding and yields an INK4-insensitive kinase, and the homologous human R24C and R24H alleles cause familial melanoma, so an R24C knockin is the appropriate tool for testing whether tumors depend on INK4 restraint or on CDK4/6 inhibitor binding. Null mice are viable but diabetic from beta cell hypoplasia and infertile, making constitutive alleles usable while conditional designs isolate tissue requirement. Cdk6 substitutes broadly, particularly in hematopoietic lineages, so single deletion understates dependency and palbociclib class resistance studies need compound alleles. Select nulls for dependency mapping, R24C knockins for oncogenic drive, and pair conditional alleles with a lineage-matched Cre.
A conventional Cdk4 knockout removes gene function in all cells that inherit the allele. It is a proven first pass for target validation when redundancy is low.
What Cdk4 Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct Cdk4 knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Cdk4-KO(2) | Knockout | KO/CKO mice | sperm cryopreservation | KO 235014 | Inquire |
Why this approach
A conventional knockout answers whether the gene is required broadly. When your target tissue is the organ of interest, a global null can still be informative if viability is acceptable and you want the simplest genotype. If the null is harsh, a floxed allele with a regional Cre is the safer long term platform.
Pricing and quotes
The Cdk4 Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Cdk4 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Cdk4 Knockout mouse models are available?
When Cdk4 Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Cdk4 knockout embryonic lethal in mice?
It depends on background and allele design. Some Cdk4 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Cdk4 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Cdk4?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.