Cdk6 Knockout mouse models
Cdk6 is the mouse ortholog of human CDK6 and encodes the second D-cyclin dependent kinase, sharing RB directed catalytic function with CDK4 and INK4 sensitivity but distinguished by dominant expression in hematopoietic stem and progenitor cells and lymphocytes, and by a kinase-independent nuclear function in which CDK6 acts as a transcriptional cofactor with STAT3, AP-1 and NF-kB to induce VEGFA and to repress p16 and p53 outputs. Chromosomal rearrangements placing CDK6 under strong enhancers occur in lymphoid malignancy, and CDK6 upregulation is a recurrent mechanism of acquired resistance to CDK4/6 inhibitors in breast cancer. Null mice are viable with mild anemia and thymic hypoplasia, so constitutive alleles are workable, but Cdk4 compensates in most nonhematopoietic tissue and single deletions mislead. Kinase-dead knockin alleles are the decisive tool here because they separate catalytic from transcriptional function, a distinction no null can make, while conditional nulls layered on Cdk4 alleles serve inhibitor resistance and hematopoietic dependency studies.
Cdk6 null animals are straightforward to genotype and phenotype when survival is acceptable. They remain a standard background for pharmacology, biomarker, and rescue studies.
What Cdk6 Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct Cdk6 knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Cdk6-KO | Knockout | KO/CKO mice | sperm cryopreservation | KO 250403 | Inquire |
Why this approach
A conventional knockout answers whether the gene is required broadly. When your target tissue is the organ of interest, a global null can still be informative if viability is acceptable and you want the simplest genotype. If the null is harsh, a floxed allele with a regional Cre is the safer long term platform.
Pricing and quotes
The Cdk6 Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Cdk6 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Cdk6 Knockout mouse models are available?
When Cdk6 Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Cdk6 knockout embryonic lethal in mice?
It depends on background and allele design. Some Cdk6 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Cdk6 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Cdk6?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.