Egfr Conditional Knockout mouse models
Egfr, the mouse ortholog of human EGFR, encodes ERBB1, a receptor tyrosine kinase whose extracellular domains I through IV adopt a tethered autoinhibited conformation until ligand binding to domains I and III exposes the domain II dimerization arm, permitting formation of an asymmetric kinase dimer in which one lobe allosterically activates the other, with autophosphorylated C-terminal tyrosines recruiting GRB2, SHC and PLC gamma to fire RAS-MAPK and PI3K-AKT. Oncogenic exon 19 deletions and L858R destabilize the inactive kinase conformation and confer TKI sensitivity, T790M and C797S confer resistance, and the vIII extracellular truncation drives glioblastoma. Critically, mouse Egfr is not bound by cetuximab and differs in domain III epitopes, so ectodomain humanization is mandatory for antibody and antibody-drug conjugate testing, while inducible mutant knockins model TKI response. Germline nulls die perinatally with strain-dependent severity, so conditional alleles plus a lineage Cre are required for adult skin, intestinal or lung studies.
Conditional deletion of Egfr limits the genetic change to the lineage you choose. That helps in oncology, immunology, and metabolic work where systemic loss would muddy the read. After you confirm Cre specificity, crossing to Egfr floxed stock yields usable cohorts.
What Egfr Conditional Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct Egfr conditional knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Egfr-Flox | Conditional Knockout | KO/CKO mice, disease model mice | embryo cryopreservation | CKO 00133 | Inquire |
Why this approach
Conditional knockout focuses the experiment on your target tissue while the rest of the animal keeps a wild type allele. That pattern mirrors somatic mutation in patients and avoids systemic compensation that can erase subtle phenotypes. It is often preferred when a germline null is lethal, weak, or confounded by developmental rescue.
Pricing and quotes
The Egfr Conditional Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Egfr allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Egfr Conditional Knockout mouse models are available?
When Egfr Conditional Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Egfr knockout embryonic lethal in mice?
It depends on background and allele design. Some Egfr germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Egfr animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Egfr?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.