Conventional / global / constitutive KO
●3 Models Available
F2 Conditional Knockout, Knockout mouse models
Browse 3 F2 mouse models including conditional knockout, knockout variants — in stock and ready to ship from ingenious targeting laboratory. Available as F2 conditional knockout mouse, F2 knockout mouse. Request a quote within 24 hours.
Why model F2
F2, ortholog of human F2, encodes prothrombin, the vitamin K dependent precursor of thrombin and the central protease of hemostasis. Prothrombinase, comprising factor Xa and factor Va on an anionic phospholipid surface, cleaves prothrombin at two sites to release the Gla and kringle containing fragment 1.2 and generate alpha-thrombin, whose substrate selection is governed by two electropositive exosites: exosite I engages fibrinogen, PAR1, and thrombomodulin, while exosite II binds heparin and GPIb alpha. Thrombin converts fibrinogen to fibrin, activates factors V, VIII, XI, and XIII along with platelet PAR1 and PAR4, and when bound to thrombomodulin switches specificity to activate protein C. Complete deficiency is lethal in mouse, with embryonic loss and neonatal hemorrhage, so hypomorphic or conditional alleles are mandatory. Note that the common human G20210A thrombophilia allele lies in the 3 prime untranslated region and raises expression rather than altering sequence, so dosage-increasing designs, not missense knockins, are the faithful mimic.
What F2 mouse models are available?
ingenious targeting laboratory offers 3 distinct F2 (F2) catalog mouse models, featuring conditional knockout and standard knockout. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
| Model Availability | Allele Class Options |
|---|---|
| 3 Ready Catalog Lines | Conditional Knockout (cKO) |
| Custom Model Generation | Constitutive Knockout (KO) |
| Advanced Modifications | Humanized, Knockin, Transgenic |
- Quality Control
- Documented Germline Transmission
- Quality Control
- Strict United States QC Guidelines
- Quality Control
- Cryo or Live Dispatch Options
F2 model types
PathAvailability
Knockout
Conditional knockout
Knockin
Humanized
Transgenic / overexpression
Prefer the order form? Order. Need an allele that is not listed? Request a quote.
F2 allele types
Knockout, conditional, knockin, humanized, and related paths for F2 (human F2). Catalog lines ship when inventory exists. Everything else is a generation quote.
Knockout
Conditional KO (floxed / loxP)
Inducible conditional KO (CreER / tet)
Tissue specific KO
Specify tissue or Cre driver on quote
Knockout first (tm1a / IKMC)
Convertible floxed allele pathway
Double / compound KO
Multi allele / compound knockout project
Large scale / BAC deletion
BAC scale deletion or targeting
Knockin
Point mutation KI
cDNA KI
Reporter KI (GFP, YFP, RFP, mCherry, tdTomato, lacZ, luciferase)
Tag KI (FLAG, HA, Myc, V5)
Conditional KI (e.g. Rosa26 LSL)
LSL or conditional expression knockin
Gene replacement KI
Humanized
Full gene replacement
Partial / domain humanized
Domain or partial humanization scope
Immune checkpoint humanized
Checkpoint IO humanization when gene is a checkpoint target
Single humanized
Double / multi humanized
Multi humanized / combination IO project
Transgenic
Random integration transgenic
Safe harbor (ROSA26, HPRT, H11, Col1a1)
Overexpression transgenic
BAC transgenic
BAC transgenic or large fragment insert
Cre / recombinase
Constitutive Cre driver
Inducible Cre (CreER / tet)
Tamoxifen or dox inducible Cre
Dual recombinase (Cre+Dre / Cre+Flp)
Dual recombinase breeding scheme
Flp driver
Flp or FRT derivative allele pairing
Reporter
Reporter knockin (gene specific)
Standalone reporter catalog (lineage tracing, constitutive, dual reporter)
Catalog reporter lines not tied to a single gene allele
Species / background
Rat KO / KI / transgenic
Specify rat on quote
Rabbit models
Specify rabbit on quote
C57BL/6 (B6J / B6N)
Background substrain on quote
BALB/c
129, NOD, NSG, FVB, CD1, DBA/2
Background strain on quote
Available F2 catalog models
3 F2 lines with QC documentation and technical support. F2 floxed mice use loxP flanked alleles for Cre dependent tissue specific knockout.
Selected publications from ingenious targeting laboratory
Peer reviewed work involving F2 from ITL supported projects.
- ZFAND6 promotes TRAF2-dependent mitophagy to restrain cGAS-STING signalingShaikh K, Bowman M, McCormick SM, Gao L, Zhang J, White J, Tawil J, Kapoor A, Arav-Boger R, Norbury CC, Harhaj EW. (2025). . iScience 1(28): 111544.
- Differential tissue specific expression of Kif23 alternative transcripts in mice with the human mutation causing congenital dyserythropoietic anemia type IIIVikberg AL, Malla S, Golovleva I. (2020). Blood Cells Mol Dis85: 102483
- Differentiated embryo chondrocyte 1 (DEC1) is a novel negative regulator of hepatic fibroblast growth factor 21 (FGF21) in aging miceFujita Y, Makishima M, Bhawal UK. (2016). Biochem Biophys Res Commun 269(3): 477-82
- SRSF2 Mutations Contribute to Myelodysplasia by Mutant-Specific Effects on Exon RecognitionKim E, Ilagan JO, Liang Y, Daubner GM, Lee SC, Ramakrishnan A, Li Y, Chung YR, Micol JB, Murphy ME, Cho H, Kim MK, Zebari AS, Aumann S, Park CY, Buonamici S, Smith PG, Deeg HJ, Lobry C, Aifantis I, Modis Y, Allain FH, Halene S, Bradley RK, Abdel-Wahab O. (2015). Cancer Cell 27(5): 617-630
- Forkhead box F2 regulation of platelet-derived growth factor and myocardin/serum response factor signaling is essential for intestinal development.Bolte C, Ren X, Tomley T, Ustiyan V, Pradhan A, Hoggatt A, Kalin TV, Herring BP, Kalinichenko VV. (2015). J Biol Chem 290: 7563-7575
Looking for a F2 mouse model?
We have 3 F2 models in stock — including Conditional Knockout, Knockout types. In stock and ready to ship this week. All models come with full QC documentation, health certificates, and dedicated technical support.
Best pricing in the industry. Get a quote in 24 hours. Our team of PhD scientists is available to help you select the right model for your research.
Order catalog model3
Models Available
2
Model Types
24 hrs
Quote Turnaround
Included
QC Documentation
About F2 Mouse Model Types
A F2 knockout mouse has the F2 gene permanently inactivated, enabling loss of function studies. A F2 conditional knockout (floxed) mouse carries loxP sites flanking a critical exon of F2, allowing Cre recombinase dependent deletion in specific tissues or at specific timepoints.
Modifications available
Jump to catalog backed pages for F2 organized by modification type. Each URL is indexable and matches common search patterns.