Fgfr2 Knockin mouse models
Fgfr2, the mouse ortholog of human FGFR2, encodes an FGF receptor whose biology is dominated by mutually exclusive splicing of exons encoding the second half of Ig domain III, producing FGFR2 IIIb in epithelium, which responds to FGF7 and FGF10 from mesenchyme, and FGFR2 IIIc in mesenchyme, which responds to epithelial FGF2, FGF8 and FGF9, establishing the reciprocal epithelial-mesenchymal loop that patterns limb, lung and craniofacial structures. Craniosynostosis alleles including S252W and P253W in Apert syndrome and cysteine substitutions in Crouzon syndrome act by broadening ligand specificity or promoting ligand-independent disulfide dimerization, while FGFR2 amplification and fusions drive gastric and intrahepatic cholangiocarcinoma. Because a conventional null is embryonic lethal from limb bud and placental failure, and because isoform-specific alleles produce entirely different phenotypes, splice-restricted alleles are the sharpest tool available. Fgfr1 and Fgfr3 compensate in skeletal contexts. Choose isoform-specific nulls for developmental patterning and conditional activating knockins for FGFR inhibitor response and resistance studies.
Targeting Fgfr2 preserves positional context compared with viral or BAC approaches. That helps when you quantify expression or map chromatin.
What Fgfr2 Knockin mouse models are available?
ingenious targeting laboratory offers 2 distinct Fgfr2 knockin catalog mouse models. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Why this approach
Knockin and humanized formats keep regulatory context at the endogenous locus. For your target tissue focused programs, that matters when expression timing, splice isoforms, or allele dosage drive biology. Random integration transgenics can still help, but targeted alleles usually give cleaner pharmacology readouts.
Pricing and quotes
The Fgfr2 Knockin lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Fgfr2 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Fgfr2 Knockin mouse models are available?
When Fgfr2 Knockin lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Fgfr2 knockout embryonic lethal in mice?
It depends on background and allele design. Some Fgfr2 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Fgfr2 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Fgfr2?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.