Fgfr3 Knockin mouse models
Unlike its paralogs, Fgfr3 acts predominantly as a negative regulator of endochondral bone growth, and the mouse ortholog of human FGFR3 encodes a receptor whose activation in growth plate chondrocytes suppresses proliferation and hypertrophic differentiation through STAT1 and MAPK, which is why gain of function alleles cause dwarfism rather than overgrowth. The transmembrane G380R substitution causes achondroplasia, K650E and K650M in the activation loop cause thanatophoric dysplasia type II and SADDAN, and null mice are viable with skeletal overgrowth and inner ear defects, exactly inverting the human mutant phenotype. In cancer, S249C and Y375C drive urothelial carcinoma through ligand-independent disulfide dimerization and FGFR3-TACC3 fusions drive glioma, both targets for erdafitinib class inhibitors. Allele logic is therefore unusually clean: knockin the specific human activating codon for skeletal dysplasia and inhibitor testing, and use nulls only when asking about growth restraint. Fgfr1 and Fgfr2 do not substitute for this inhibitory function, so single-locus knockins are interpretable.
Knockin designs at Fgfr3 support head to head comparisons between wild type and modified alleles because regulatory DNA stays in place.
What Fgfr3 Knockin mouse models are available?
ingenious targeting laboratory offers 3 distinct Fgfr3 knockin catalog mouse models. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Why this approach
Knockin and humanized formats keep regulatory context at the endogenous locus. For your target tissue focused programs, that matters when expression timing, splice isoforms, or allele dosage drive biology. Random integration transgenics can still help, but targeted alleles usually give cleaner pharmacology readouts.
Pricing and quotes
The Fgfr3 Knockin lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Fgfr3 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Fgfr3 Knockin mouse models are available?
When Fgfr3 Knockin lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Fgfr3 knockout embryonic lethal in mice?
It depends on background and allele design. Some Fgfr3 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Fgfr3 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Fgfr3?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.