Gabra4 Knockout mouse models
Gabra4 encodes the α4 subunit, ortholog of human GABRA4, which partners preferentially with δ rather than γ2 and localizes to extrasynaptic and perisynaptic membranes of dentate granule cells, thalamus and striatum, where α4βδ receptors mediate high-affinity tonic GABA current rather than phasic inhibition. These receptors carry an arginine at the position equivalent to α1 His101, rendering them benzodiazepine-insensitive, while remaining exquisitely sensitive to neurosteroids such as allopregnanolone, to low-dose ethanol, and to the superagonist gaboxadol, which is the pharmacological signature that defines the subunit. Expression is plastic, rising sharply after stress, ethanol withdrawal and across the ovarian cycle, so phenotyping must control for hormonal state. Constitutive nulls are viable and lose gaboxadol-sensitive tonic current, so a conditional null with Pomc-Cre or a dentate driver localizes the contribution, whereas δ subunit deletion produces overlapping phenotypes; measure tonic current amplitude directly rather than inferring from behavior.
Global Gabra4 deletion answers broad mechanism questions quickly. If timing or site matters, conditional alleles are a natural next step after the null is characterized.
What Gabra4 Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct Gabra4 knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Gabra4-KO | Knockout | KO/CKO mice, disease model mice | sperm cryopreservation | KO 190572 | Inquire |
Why this approach
A conventional knockout answers whether the gene is required broadly. When your target tissue is the organ of interest, a global null can still be informative if viability is acceptable and you want the simplest genotype. If the null is harsh, a floxed allele with a regional Cre is the safer long term platform.
Pricing and quotes
The Gabra4 Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Gabra4 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Gabra4 Knockout mouse models are available?
When Gabra4 Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Gabra4 knockout embryonic lethal in mice?
It depends on background and allele design. Some Gabra4 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Gabra4 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Gabra4?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.