Gabrb1 Knockout mouse models
Gabrb1 encodes the β1 GABA-A receptor subunit, ortholog of human GABRB1, one of three β isoforms that contribute the principal side of the orthosteric GABA binding site at the β and α interface and that carry the transmembrane residues determining sensitivity to etomidate, propofol and loreclezole. Relative to β2 and β3 it is the least abundant, with expression concentrated in hippocampus, septum and specific hypothalamic and brainstem nuclei, and it is the sole β subunit in the imprinted-adjacent chromosome 4p12 cluster with GABRA2, GABRA4 and GABRG1. De novo missense variants in the M2 pore-lining segment cause developmental and epileptic encephalopathy by increasing spontaneous channel opening, a gain-of-function mechanism that argues for a patient knockin rather than a null. Because β2 and β3 substitute broadly during assembly, a single Gabrb1 deletion is frequently silent; plan compound conditional alleles, quantify pentamer composition biochemically, and read out anesthetic sensitivity alongside EEG.
Global Gabrb1 deletion answers broad mechanism questions quickly. If timing or site matters, conditional alleles are a natural next step after the null is characterized.
What Gabrb1 Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct Gabrb1 knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Gabrb1-KO | Knockout | KO/CKO mice | embryo cryopreservation | KO 190575 | Inquire |
Why this approach
A conventional knockout answers whether the gene is required broadly. When your target tissue is the organ of interest, a global null can still be informative if viability is acceptable and you want the simplest genotype. If the null is harsh, a floxed allele with a regional Cre is the safer long term platform.
Pricing and quotes
The Gabrb1 Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Gabrb1 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Gabrb1 Knockout mouse models are available?
When Gabrb1 Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Gabrb1 knockout embryonic lethal in mice?
It depends on background and allele design. Some Gabrb1 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Gabrb1 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Gabrb1?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.