Gdf3 Conditional Knockout mouse models
Gdf3, ortholog of human GDF3, encodes a divergent TGF-beta ligand expressed in the inner cell mass, embryonic stem cells, and adipose tissue that behaves context-dependently, either signaling like Nodal through ActRIIB and ALK4 with an EGF-CFC coreceptor, or acting as an extracellular BMP antagonist that sequesters BMP4. This dual character explains why loss produces partially penetrant defects in anterior visceral endoderm positioning and mesoderm patterning rather than one clean phenotype, and why human variants segregate with microphthalmia, coloboma, and Klippel-Feil vertebral fusion. Conditional nulls address tissue-specific requirements in visceral endoderm versus adipocytes, knocking in patient missense alleles distinguishes a hypomorphic ligand from a true antimorph, and humanization supports biochemical assays of receptor and BMP binding. Because Gdf1 is the closest paralog and both ligands feed Nodal-type signaling, interpret single-locus phenotypes cautiously and build compound Gdf1 alleles whenever laterality or axial patterning is the endpoint.
Conditional deletion of Gdf3 limits the genetic change to the lineage you choose. That helps in oncology, immunology, and metabolic work where systemic loss would muddy the read. After you confirm Cre specificity, crossing to Gdf3 floxed stock yields usable cohorts.
What Gdf3 Conditional Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct Gdf3 conditional knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Gdf3-Flox | Conditional Knockout | KO/CKO mice | live | CKO 240719 | Inquire |
Why this approach
Conditional knockout focuses the experiment on your target tissue while the rest of the animal keeps a wild type allele. That pattern mirrors somatic mutation in patients and avoids systemic compensation that can erase subtle phenotypes. It is often preferred when a germline null is lethal, weak, or confounded by developmental rescue.
Pricing and quotes
The Gdf3 Conditional Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Gdf3 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Gdf3 Conditional Knockout mouse models are available?
When Gdf3 Conditional Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Gdf3 knockout embryonic lethal in mice?
It depends on background and allele design. Some Gdf3 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Gdf3 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Gdf3?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.