Gria1 Knockout mouse models
Encoding the GluA1 subunit of AMPA-type glutamate receptors, Gria1 is the mouse ortholog of human GRIA1 and contributes the activity-regulated, long cytoplasmic tail arm of tetrameric receptors whose trafficking underlies hippocampal long-term potentiation. GluA1 remains unedited at the Q/R site, so GluA2-lacking assemblies are calcium permeable and inwardly rectifying, and its tail carries the S831 CaMKII/PKC and S845 PKA phosphosites plus PDZ binding for SAP97, while TARP auxiliary subunits such as Cacng8 and stargazin govern surface delivery and channel kinetics. Null mice are viable and fertile but show loss of extrasynaptic AMPA current, deficient CA1 LTP, working memory impairment, and altered locomotor sensitization, making germline deletion informative. Conditional alleles isolate circuit contributions, phosphosite knockins test whether specific residues are required for plasticity, and humanization supports antibody or modulator work. Because Gria2 through Gria4 form heteromers that partially buffer subunit loss, plan compound crosses before attributing phenotypes to a single locus.
A conventional Gria1 knockout removes gene function in all cells that inherit the allele. It is a proven first pass for target validation when redundancy is low.
What Gria1 Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct Gria1 knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Gria1-KO | Knockout | KO/CKO mice | embryo cryopreservation | KO 190582 | Inquire |
Why this approach
A conventional knockout answers whether the gene is required broadly. When your target tissue is the organ of interest, a global null can still be informative if viability is acceptable and you want the simplest genotype. If the null is harsh, a floxed allele with a regional Cre is the safer long term platform.
Pricing and quotes
The Gria1 Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Gria1 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Gria1 Knockout mouse models are available?
When Gria1 Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Gria1 knockout embryonic lethal in mice?
It depends on background and allele design. Some Gria1 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Gria1 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Gria1?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.