Grin2b Knockout mouse models
GluN2B, encoded by Grin2b, the mouse ortholog of human GRIN2B, is the dominant NMDA receptor GluN2 subunit in embryonic and early postnatal brain, generating currents with slow deactivation and high charge transfer, and it is uniquely blocked by ifenprodil and related amino-terminal domain antagonists. Its exceptionally long cytoplasmic tail binds CaMKII with high affinity and scaffolds PSD-95 and SynGAP, so GluN2B is not merely a pore component but a signaling hub for synaptic plasticity and spine maturation. Constitutive nulls die perinatally with failed suckling and absent hippocampal long-term depression, which mandates floxed alleles combined with CaMKIIa-Cre or Emx1-Cre for adult work. Human GRIN2B variants cause intellectual disability and epileptic encephalopathy through both reduced current and gating gain of function, favoring targeted knockins, while tail-swap alleles separate ion flux from CaMKII recruitment. GluN2A upregulation partially masks loss, so measure ifenprodil-sensitive current directly rather than inferring composition.
Grin2b null animals are straightforward to genotype and phenotype when survival is acceptable. They remain a standard background for pharmacology, biomarker, and rescue studies.
What Grin2b Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct Grin2b knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Grin2b-KO | Knockout | KO/CKO mice | sperm cryopreservation | KO 250434 | Inquire |
Why this approach
A conventional knockout answers whether the gene is required broadly. When your target tissue is the organ of interest, a global null can still be informative if viability is acceptable and you want the simplest genotype. If the null is harsh, a floxed allele with a regional Cre is the safer long term platform.
Pricing and quotes
The Grin2b Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Grin2b allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Grin2b Knockout mouse models are available?
When Grin2b Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Grin2b knockout embryonic lethal in mice?
It depends on background and allele design. Some Grin2b germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Grin2b animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Grin2b?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.