Igf2r Knockout mouse models
The mouse Igf2r locus, ortholog of human IGF2R, encodes the cation-independent mannose-6-phosphate receptor, a large type I transmembrane protein whose extracytoplasmic repeats bind both mannose-6-phosphate-tagged lysosomal hydrolases, delivered from the trans-Golgi network to endosomes, and IGF-2, which it internalizes for degradation, making it a ligand clearance sink rather than a signaling receptor. In mouse the gene is maternally expressed and imprinted through the intronic Airn noncoding RNA, a regulatory feature absent in humans that must be considered before extrapolating dosage effects. Maternal-allele nulls are lethal with generalized overgrowth and cardiac defects that are rescued by concurrent Igf2 deletion, evidence that the phenotype reflects excess IGF-2 rather than lysosomal misrouting. Conditional alleles therefore matter for postnatal work, while separation-of-function knockins that ablate the IGF-2 binding repeat while preserving hydrolase sorting cleanly split the two activities. Choose endpoints accordingly, including circulating IGF-2, lysosomal enzyme secretion, and cardiac and placental morphometry.
Igf2r null animals are straightforward to genotype and phenotype when survival is acceptable. They remain a standard background for pharmacology, biomarker, and rescue studies.
What Igf2r Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct Igf2r knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Igf2r-KO | Knockout | KO/CKO mice | In production — inquire | KO 253248 | Inquire |
Why this approach
A conventional knockout answers whether the gene is required broadly. When your target tissue is the organ of interest, a global null can still be informative if viability is acceptable and you want the simplest genotype. If the null is harsh, a floxed allele with a regional Cre is the safer long term platform.
Pricing and quotes
The Igf2r Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Igf2r allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Igf2r Knockout mouse models are available?
When Igf2r Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Igf2r knockout embryonic lethal in mice?
It depends on background and allele design. Some Igf2r germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Igf2r animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Igf2r?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.