Il2 Knockin mouse models
Il2 is the mouse ortholog of human IL2 and encodes a four alpha helix bundle cytokine that assembles a trimeric receptor of IL-2Ralpha, IL-2Rbeta, and the common gamma chain, activating JAK1 and JAK3 with STAT5 to control regulatory T cell fitness, effector CD8 expansion, and activation induced cell death. Because high-affinity capture depends on constitutive CD25 on regulatory T cells, interleukin 2 availability is a tolerance checkpoint, and germline Il2 or Il2ra nulls develop lethal lymphoproliferation with autoimmune hemolytic anemia and colitis rather than immunodeficiency, a phenotype that constrains any breeding scheme. Allele class should follow the endpoint: conditional deletion in CD4 T cells asks who supplies the cytokine, knockins encoding not-alpha or CD25-biased muteins model low dose interleukin 2 therapy, and humanization supports aldesleukin and engineered agonist pharmacology. Plan drivers early, since Cd4-Cre, Foxp3-Cre, and inducible CreERT2 lines give very different windows on tolerance versus antitumor expansion.
Il2 knockin models place a defined sequence at the endogenous locus. Expression stays under native regulation, which matters for reporters, tags, and precise allele swaps.
What Il2 Knockin mouse models are available?
ingenious targeting laboratory offers 2 distinct Il2 knockin catalog mouse models. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Why this approach
Knockin and humanized formats keep regulatory context at the endogenous locus. For your target tissue focused programs, that matters when expression timing, splice isoforms, or allele dosage drive biology. Random integration transgenics can still help, but targeted alleles usually give cleaner pharmacology readouts.
Pricing and quotes
The Il2 Knockin lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Il2 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Il2 Knockin mouse models are available?
When Il2 Knockin lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Il2 knockout embryonic lethal in mice?
It depends on background and allele design. Some Il2 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Il2 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Il2?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.