Irf5 Knockout mouse models
Irf5 is the mouse ortholog of human IRF5 and encodes an interferon regulatory factor acting mainly downstream of MyD88 coupled TLR7 and TLR9 signaling, where TRAF6 dependent ubiquitination and IKKbeta mediated phosphorylation drive nuclear translocation and transcription of interleukin 6, interleukin 12, interleukin 23, and TNF, making it a determinant of inflammatory macrophage polarization and plasma cell differentiation rather than of type I interferon amplification. Human IRF5 haplotypes are among the strongest non-HLA risk factors for systemic lupus erythematosus, giving clear rationale for both loss-of-function and expression level alleles. One technical caveat dominates this locus: several widely used Irf5 knockout lines carried a closely linked spontaneous Dock2 mutation that independently distorts lymphocyte development and migration, so new projects should use a freshly targeted conditional allele and genotype Dock2 explicitly. Pair the flox with Lyz2-Cre or Cd19-Cre to separate myeloid from B lineage contributions, and humanize when antibody or degrader pharmacology is the endpoint.
Irf5 null animals are straightforward to genotype and phenotype when survival is acceptable. They remain a standard background for pharmacology, biomarker, and rescue studies.
What Irf5 Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct Irf5 knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Irf5-KO | Knockout | KO/CKO mice | sperm cryopreservation | KO 205041 | Inquire |
Why this approach
A conventional knockout answers whether the gene is required broadly. When your target tissue is the organ of interest, a global null can still be informative if viability is acceptable and you want the simplest genotype. If the null is harsh, a floxed allele with a regional Cre is the safer long term platform.
Pricing and quotes
The Irf5 Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Irf5 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Irf5 Knockout mouse models are available?
When Irf5 Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Irf5 knockout embryonic lethal in mice?
It depends on background and allele design. Some Irf5 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Irf5 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Irf5?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.