Jag1 Knockout mouse models
Jag1 is the mouse ortholog of human JAG1 and encodes a single-pass Notch ligand built from a DSL domain, sixteen EGF-like repeats, and a cysteine-rich region, which engages NOTCH1 through NOTCH4 in trans and requires mechanical force to expose the receptor S2 site for ADAM10, licensing gamma-secretase cleavage and NICD release, with Fringe glycosylation of the receptor tuning Jagged versus Delta-like preference and cis expression acting inhibitory. Heterozygous human loss of function causes Alagille syndrome with bile duct paucity, cardiac outflow defects, butterfly vertebrae, and posterior embryotoxon, while nulls die near E10.5 from failed vascular remodeling. Conditional deletion in portal mesenchyme, endothelium, or arterial mural cells is therefore mandatory, Alagille missense knockins distinguish trafficking-defective from binding-defective ligand, and humanization enables antibody and decoy testing. Jag2, Dll1, and Dll4 supply overlapping ligand activity, and sensitized Notch2 hypomorph backgrounds are often required to reveal biliary phenotypes.
Global Jag1 deletion answers broad mechanism questions quickly. If timing or site matters, conditional alleles are a natural next step after the null is characterized.
What Jag1 Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct Jag1 knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Jag1-KO | Knockout | KO/CKO mice | embryo cryopreservation | KO 205057 | Inquire |
Why this approach
A conventional knockout answers whether the gene is required broadly. When your target tissue is the organ of interest, a global null can still be informative if viability is acceptable and you want the simplest genotype. If the null is harsh, a floxed allele with a regional Cre is the safer long term platform.
Pricing and quotes
The Jag1 Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Jag1 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Jag1 Knockout mouse models are available?
When Jag1 Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Jag1 knockout embryonic lethal in mice?
It depends on background and allele design. Some Jag1 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Jag1 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Jag1?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.