Jag2 Knockout mouse models
Jag2, the mouse ortholog of human JAG2, encodes a second Jagged-class Notch ligand and presents a DSL domain with EGF repeats to NOTCH1 through NOTCH3, triggering force-dependent ADAM10 and gamma-secretase processing and NICD-driven transcription with RBPJ and MAML. Its non-redundant requirements concentrate in thymic epithelium and gamma delta T cell development, the limb apical ectodermal ridge, tooth, and secondary palate, and nulls die perinatally with cleft palate, limb syndactyly, and thymic hypoplasia, while biallelic human variants cause a recessive muscular dystrophy tied to satellite cell Notch signaling. Given perinatal lethality, conditional deletion is the practical design for postnatal thymus, muscle regeneration, or skin work, patient missense knockins test whether variants impair surface presentation or receptor binding, and humanization supports antibody pharmacology. Jag1 overlaps substantially in epithelial and vascular contexts, so compound conditional alleles paired with a lineage-validated Cre driver prevent masked phenotypes.
A conventional Jag2 knockout removes gene function in all cells that inherit the allele. It is a proven first pass for target validation when redundancy is low.
What Jag2 Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct Jag2 knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Jag2-KO | Knockout | KO/CKO mice | sperm cryopreservation | KO 250114 | Inquire |
Why this approach
A conventional knockout answers whether the gene is required broadly. When your target tissue is the organ of interest, a global null can still be informative if viability is acceptable and you want the simplest genotype. If the null is harsh, a floxed allele with a regional Cre is the safer long term platform.
Pricing and quotes
The Jag2 Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Jag2 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Jag2 Knockout mouse models are available?
When Jag2 Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Jag2 knockout embryonic lethal in mice?
It depends on background and allele design. Some Jag2 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Jag2 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Jag2?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.