Lrrk2 Knockin mouse models
Lrrk2, the mouse ortholog of human LRRK2, encodes a large multidomain protein combining armadillo, ankyrin, and leucine-rich repeats with a ROC GTPase domain, a COR linker, a serine/threonine kinase domain, and a WD40 tail, functioning as a dimeric kinase recruited to damaged or stressed membranes. Its validated substrates are a subset of Rab GTPases, including Rab8a, Rab10, and Rab29, phosphorylated at a conserved threonine in the switch II motif, which blocks GDI binding and reroutes membrane trafficking, ciliogenesis, and lysosomal repair. The G2019S mutation in the kinase domain and R1441C/G in the ROC domain increase Rab10 phosphorylation and cause autosomal dominant Parkinson disease, so knockin alleles, not deletion, reproduce the disease mechanism, while nulls are viable but develop kidney and lung lamellar body pathology that constrains interpretation and mirrors the on-target liability of kinase inhibitors. Kinase-dead knockins separate scaffolding from catalysis; use phospho-Rab10 as the pharmacodynamic endpoint.
Lrrk2 knockin models place a defined sequence at the endogenous locus. Expression stays under native regulation, which matters for reporters, tags, and precise allele swaps.
What Lrrk2 Knockin mouse models are available?
ingenious targeting laboratory offers 1 distinct Lrrk2 knockin catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Lrrk2-P1446L | Knockin | point mutantion mice, disease model mice | embryo cryopreservation | KI 215098 | Inquire |
Why this approach
Knockin and humanized formats keep regulatory context at the endogenous locus. For your target tissue focused programs, that matters when expression timing, splice isoforms, or allele dosage drive biology. Random integration transgenics can still help, but targeted alleles usually give cleaner pharmacology readouts.
Pricing and quotes
The Lrrk2 Knockin lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Lrrk2 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Lrrk2 Knockin mouse models are available?
When Lrrk2 Knockin lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Lrrk2 knockout embryonic lethal in mice?
It depends on background and allele design. Some Lrrk2 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Lrrk2 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Lrrk2?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.