Nrg1 Knockout mouse models
Nrg1, the mouse ortholog of human NRG1, encodes neuregulin 1, a family of EGF-domain ligands generated from a single locus through multiple promoters and splice choices that yield type I and type II isoforms with immunoglobulin-like amino termini, type III isoforms with a cysteine-rich transmembrane region that signal juxtacrine, and alpha or beta EGF variants with different receptor affinity. Most isoforms are made as transmembrane precursors cleaved by Bace1 and Adam17 to release the EGF domain, which binds Erbb3 or Erbb4 and drives heterodimerization with Erbb2 to activate PI3K and Erk. Homozygous nulls die at mid-gestation from failed cardiac trabeculation, so isoform-specific conditional exon targeting is the design that isolates type III-dependent Schwann cell myelination or type I-dependent cardiac and interneuron phenotypes. Human NRG1 fusions in lung adenocarcinoma justify fusion knockins, and Nrg2, Nrg3, and Nrg4 provide overlapping Erbb ligands that can mask single-gene loss.
A conventional Nrg1 knockout removes gene function in all cells that inherit the allele. It is a proven first pass for target validation when redundancy is low.
What Nrg1 Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct Nrg1 knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Nrg1-KO | Knockout | KO/CKO mice | sperm cryopreservation | KO 233852 | Inquire |
Why this approach
A conventional knockout answers whether the gene is required broadly. When your target tissue is the organ of interest, a global null can still be informative if viability is acceptable and you want the simplest genotype. If the null is harsh, a floxed allele with a regional Cre is the safer long term platform.
Pricing and quotes
The Nrg1 Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Nrg1 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Nrg1 Knockout mouse models are available?
When Nrg1 Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Nrg1 knockout embryonic lethal in mice?
It depends on background and allele design. Some Nrg1 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Nrg1 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Nrg1?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.