Conventional / global / constitutive KO
●2 Models Available
Raf1 Conditional Knockout, Knockin mouse models
Browse 2 Raf1 mouse models including conditional knockout, knockin variants from ingenious targeting laboratory. Available as Raf1 conditional knockout mouse, Raf1 knockin mouse. Contact us for availability and fast turnaround.
Why model Raf1
Encoding CRAF, Raf1 is the mouse ortholog of human RAF1 and produces a serine and threonine kinase held autoinhibited by 14-3-3 binding at phospho-Ser259 and by its cysteine-rich domain until RAS-GTP engages the RAS-binding domain at the membrane, after which SHOC2 and PP1 dephosphorylation, Ser338 phosphorylation and side-to-side dimerization with BRAF generate an active kinase that phosphorylates MEK1 and MEK2. Germline Noonan syndrome substitutions such as S257L and S259F disrupt the 14-3-3 brake and cause hypertrophic cardiomyopathy, providing a knockin design distinct from a null, and kinase-independent functions in ASK1 and ROK alpha regulation argue for kinase-dead knockins alongside deletion. Constitutive deletion is embryonic lethal with widespread apoptosis on most backgrounds, so conditional nulls are needed for adult work. Araf and Braf occupy the same dimer interface and partially compensate, so interpret single-locus loss against dimerization-competent paralog expression in the target tissue.
What Raf1 mouse models are available?
ingenious targeting laboratory offers 2 distinct Raf1 (RAF1) catalog mouse models, featuring conditional knockout and knockin. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
| Model Availability | Allele Class Options |
|---|---|
| 2 Ready Catalog Lines | Conditional Knockout (cKO) |
| Custom Model Generation | Constitutive Knockout (KO) on request |
| Advanced Modifications | Humanized, Knockin, Transgenic |
- Quality Control
- Documented Germline Transmission
- Quality Control
- Strict United States QC Guidelines
- Quality Control
- Cryo or Live Dispatch Options
Raf1 model types
PathAvailability
Knockout
Conditional knockout
Knockin
Humanized
Transgenic / overexpression
Prefer the order form? Order. Need an allele that is not listed? Request a quote.
Raf1 allele types
Knockout, conditional, knockin, humanized, and related paths for Raf1 (human RAF1). Catalog lines ship when inventory exists. Everything else is a generation quote.
Knockout
Conditional KO (floxed / loxP)
Inducible conditional KO (CreER / tet)
Tissue specific KO
Specify tissue or Cre driver on quote
Knockout first (tm1a / IKMC)
Convertible floxed allele pathway
Double / compound KO
Multi allele / compound knockout project
Large scale / BAC deletion
BAC scale deletion or targeting
Knockin
Point mutation KI
cDNA KI
Reporter KI (GFP, YFP, RFP, mCherry, tdTomato, lacZ, luciferase)
Tag KI (FLAG, HA, Myc, V5)
Conditional KI (e.g. Rosa26 LSL)
LSL or conditional expression knockin
Gene replacement KI
Humanized
Full gene replacement
Partial / domain humanized
Domain or partial humanization scope
Immune checkpoint humanized
Checkpoint IO humanization when gene is a checkpoint target
Single humanized
Double / multi humanized
Multi humanized / combination IO project
Transgenic
Random integration transgenic
Safe harbor (ROSA26, HPRT, H11, Col1a1)
Overexpression transgenic
BAC transgenic
BAC transgenic or large fragment insert
Cre / recombinase
Constitutive Cre driver
Inducible Cre (CreER / tet)
Tamoxifen or dox inducible Cre
Dual recombinase (Cre+Dre / Cre+Flp)
Dual recombinase breeding scheme
Flp driver
Flp or FRT derivative allele pairing
Reporter
Reporter knockin (gene specific)
Standalone reporter catalog (lineage tracing, constitutive, dual reporter)
Catalog reporter lines not tied to a single gene allele
Species / background
Rat KO / KI / transgenic
Specify rat on quote
Rabbit models
Specify rabbit on quote
C57BL/6 (B6J / B6N)
Background substrain on quote
BALB/c
129, NOD, NSG, FVB, CD1, DBA/2
Background strain on quote
Available Raf1 catalog models
2 Raf1 lines with QC documentation and technical support. Raf1 floxed mice use loxP flanked alleles for Cre dependent tissue specific knockout.
Looking for a Raf1 mouse model?
We have 2 Raf1 models available — including Conditional Knockout, Knockin types. Contact us today for current availability and our fastest turnaround options. All models come with full QC documentation and technical support.
Best pricing in the industry. Get a quote in 24 hours. Our team of PhD scientists is available to help you select the right model for your research.
Order catalog model2
Models Available
2
Model Types
24 hrs
Quote Turnaround
Included
QC Documentation
About Raf1 Mouse Model Types
A Raf1 conditional knockout (floxed) mouse carries loxP sites flanking a critical exon of Raf1, allowing Cre recombinase dependent deletion in specific tissues or at specific timepoints. Raf1 knockin models carry a precisely inserted sequence at the Raf1 locus, useful for reporter, tag, or humanization studies.
Modifications available
Jump to catalog backed pages for Raf1 organized by modification type. Each URL is indexable and matches common search patterns.