Rb1 Knockin mouse models
The prototypic tumor suppressor Rb1, ortholog of human RB1, encodes a pocket protein whose A and B domains form a cleft that binds the transactivation domain of E2F1 through E2F3 and, through a separate LXCXE-binding groove, recruits histone deacetylases and chromatin modifiers as well as viral oncoproteins including E7 and large T antigen. Sequential phosphorylation by cyclin D with CDK4 or CDK6 and then cyclin E with CDK2 releases E2F and licenses S phase entry, the axis targeted by palbociclib, and biallelic loss causes retinoblastoma and is pervasive in small cell lung cancer. Germline deletion is embryonic lethal from placental and erythroid defects, so conditional nulls are standard, and phosphorylation site or LXCXE-cleft knockins separate CDK regulation from chromatin recruitment. Critically, the paralogs Rbl1 and Rbl2 compensate robustly in mouse retina and elsewhere, so compound conditional genetics is usually required to reproduce the human tumor spectrum.
Targeting Rb1 preserves positional context compared with viral or BAC approaches. That helps when you quantify expression or map chromatin.
What Rb1 Knockin mouse models are available?
ingenious targeting laboratory offers 1 distinct Rb1 knockin catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Rb1-Flox/Trp53-Flox/R26-CAG-LSL-Luc-2A-EGFP | Knockin | KO/CKO mice, over expression mice, fluorescent mouse | embryo cryopreservation | KI 234513 | Inquire |
Why this approach
Knockin and humanized formats keep regulatory context at the endogenous locus. For your target tissue focused programs, that matters when expression timing, splice isoforms, or allele dosage drive biology. Random integration transgenics can still help, but targeted alleles usually give cleaner pharmacology readouts.
Pricing and quotes
The Rb1 Knockin lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Rb1 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Rb1 Knockin mouse models are available?
When Rb1 Knockin lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Rb1 knockout embryonic lethal in mice?
It depends on background and allele design. Some Rb1 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Rb1 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Rb1?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.