Slc6a1 Knockin mouse models
GABA reuptake at cortical synapses is mediated by Slc6a1, the mouse ortholog of human SLC6A1, encoding GAT-1, a twelve-transmembrane sodium and chloride dependent symporter of the SLC6 family with the LeuT fold, expressed on GABAergic axon terminals and on astrocytes, where it terminates phasic inhibition and constrains ambient GABA and tonic conductance. It is the pharmacological target of tiagabine. Heterozygous SLC6A1 missense and truncating variants cause myoclonic atonic epilepsy, absence seizures, autism, and intellectual disability, and importantly many missense alleles act through misfolding and ER retention rather than pure haploinsufficiency, which strongly favors patient knockin over simple null when mechanism is the question. Slc6a1 knockout mice are viable with tremor, ataxia, elevated tonic inhibition, and altered seizure susceptibility. Use conditional alleles with neuronal versus astrocytic Cre drivers to separate the two GAT-1 pools, and humanized alleles when chaperone or oligonucleotide therapeutics are tested.
Knockin designs at Slc6a1 support head to head comparisons between wild type and modified alleles because regulatory DNA stays in place.
What Slc6a1 Knockin mouse models are available?
ingenious targeting laboratory offers 2 distinct Slc6a1 knockin catalog mouse models. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Why this approach
Knockin and humanized formats keep regulatory context at the endogenous locus. For your target tissue focused programs, that matters when expression timing, splice isoforms, or allele dosage drive biology. Random integration transgenics can still help, but targeted alleles usually give cleaner pharmacology readouts.
Pricing and quotes
The Slc6a1 Knockin lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Slc6a1 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Slc6a1 Knockin mouse models are available?
When Slc6a1 Knockin lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Slc6a1 knockout embryonic lethal in mice?
It depends on background and allele design. Some Slc6a1 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Slc6a1 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Slc6a1?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.