Conventional / global / constitutive KO
●3 Models Available
Smo Conditional Knockout, Knockout mouse models
Browse 3 Smo mouse models including conditional knockout, knockout variants from ingenious targeting laboratory. Available as Smo conditional knockout mouse, Smo knockout mouse. Contact us for availability and fast turnaround.
Why model Smo
Smo, the ortholog of human SMO, encodes a class F G protein coupled receptor with a seven-transmembrane bundle plus an extracellular cysteine-rich domain that binds cholesterol, the activating sterol whose access is restricted by PTCH1 until Hedgehog engagement. Activation drives SMO accumulation in the primary cilium, phosphorylation by GRK2 and CK1, suppression of PKA-dependent GLI3 processing and release of GLI2 activator. Oncogenic substitutions such as the SmoM2 allele and the vismodegib resistance mutation D473H make Cre-inducible knockins, particularly the lox-stop-lox SmoM2 allele, the standard tools for driving pathway activation in a defined lineage and for modeling acquired inhibitor resistance. Germline deletion is embryonic lethal with cardiac and left to right asymmetry defects, so conditional nulls are required to test ligand dependence in adult tissues. Match allele class to question: activating knockin for tumor initiation and drug resistance, conditional null for pathway requirement and ciliary trafficking.
What Smo mouse models are available?
ingenious targeting laboratory offers 3 distinct Smo (SMO) catalog mouse models, featuring conditional knockout and standard knockout. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
| Model Availability | Allele Class Options |
|---|---|
| 3 Ready Catalog Lines | Conditional Knockout (cKO) |
| Custom Model Generation | Constitutive Knockout (KO) |
| Advanced Modifications | Humanized, Knockin, Transgenic |
- Quality Control
- Documented Germline Transmission
- Quality Control
- Strict United States QC Guidelines
- Quality Control
- Cryo or Live Dispatch Options
Smo model types
PathAvailability
Knockout
Conditional knockout
Knockin
Humanized
Transgenic / overexpression
Prefer the order form? Order. Need an allele that is not listed? Request a quote.
Smo allele types
Knockout, conditional, knockin, humanized, and related paths for Smo (human SMO). Catalog lines ship when inventory exists. Everything else is a generation quote.
Knockout
Conditional KO (floxed / loxP)
Inducible conditional KO (CreER / tet)
Tissue specific KO
Specify tissue or Cre driver on quote
Knockout first (tm1a / IKMC)
Convertible floxed allele pathway
Double / compound KO
Multi allele / compound knockout project
Large scale / BAC deletion
BAC scale deletion or targeting
Knockin
Point mutation KI
cDNA KI
Reporter KI (GFP, YFP, RFP, mCherry, tdTomato, lacZ, luciferase)
Tag KI (FLAG, HA, Myc, V5)
Conditional KI (e.g. Rosa26 LSL)
LSL or conditional expression knockin
Gene replacement KI
Humanized
Full gene replacement
Partial / domain humanized
Domain or partial humanization scope
Immune checkpoint humanized
Checkpoint IO humanization when gene is a checkpoint target
Single humanized
Double / multi humanized
Multi humanized / combination IO project
Transgenic
Random integration transgenic
Safe harbor (ROSA26, HPRT, H11, Col1a1)
Overexpression transgenic
BAC transgenic
BAC transgenic or large fragment insert
Cre / recombinase
Constitutive Cre driver
Inducible Cre (CreER / tet)
Tamoxifen or dox inducible Cre
Dual recombinase (Cre+Dre / Cre+Flp)
Dual recombinase breeding scheme
Flp driver
Flp or FRT derivative allele pairing
Reporter
Reporter knockin (gene specific)
Standalone reporter catalog (lineage tracing, constitutive, dual reporter)
Catalog reporter lines not tied to a single gene allele
Species / background
Rat KO / KI / transgenic
Specify rat on quote
Rabbit models
Specify rabbit on quote
C57BL/6 (B6J / B6N)
Background substrain on quote
BALB/c
129, NOD, NSG, FVB, CD1, DBA/2
Background strain on quote
Available Smo catalog models
3 Smo lines with QC documentation and technical support. Smo floxed mice use loxP flanked alleles for Cre dependent tissue specific knockout.
Selected publications from ingenious targeting laboratory
Peer reviewed work involving Smo (SMO) from ITL supported projects.
- Microbiota-dependent formylated peptide receptor (Fpr1/2) signaling regulates enteric nervous system development and gastrointestinal motility in miceChandrasekharan B, Wu H, Smoller C, Kim J, Wolfarth AA, Eboka R, Boyer D, Metzger AJ, Addis CR, Liu K, Srinivasan S, Macpherson AJ, Jones RM, Neish AS. (2025). Cell Mol Gastroenterol Hepatol. 12(19): 101624.
- Thrombin-activated interleukin-1α drives atherogenesis, but also promotes vascular smooth muscle cell proliferation and collagen production.Burzynski LC, Morales-Maldonado A, Rodgers A, Kitt LA, Humphry M, Figg N, Bennett MR, Clarke MCH. (2023). Cardiovasc Res 119(12): 2179-89
- NOXA1-dependent NADPH Oxidase Regulates Redox Signaling and Phenotype of Vascular Smooth Muscle Cell During AtherogenesisVendrov AE, Sumida A, Canugovi C, Lozhkin A, Hayami T, Madamanchi NR, Runge MS. (2019). Redox Biol 21:101063
- Identification of small proline-rich repeat protein 3 as a novel atheroprotective factor that promotes adaptive Akt signaling in vascular smooth muscle cellsSegedy AK, Pyle AL, Li B, Zhang Y, Babaev VR, Jat P, Fazio S, Atkinson JB, Linton MF, Young PP. (2014). Arterioscler Thromb Vasc Biol 34(12): 2527-36
- Regulation of 130-kDa smooth muscle myosin light chain kinase expression by an intronic CArG element.Chen M, Zhang W, Lu X, Hoggatt AM, Gunst SJ, Kassab GS, Tune JD, Herring BP. (2013). J Biol Chem 288: 34647-34657
Looking for a Smo mouse model?
We have 3 Smo models available — including Conditional Knockout, Knockout types. Contact us today for current availability and our fastest turnaround options. All models come with full QC documentation and technical support.
Best pricing in the industry. Get a quote in 24 hours. Our team of PhD scientists is available to help you select the right model for your research.
Order catalog model3
Models Available
2
Model Types
24 hrs
Quote Turnaround
Included
QC Documentation
About Smo Mouse Model Types
A Smo knockout mouse has the Smo gene permanently inactivated, enabling loss of function studies. A Smo conditional knockout (floxed) mouse carries loxP sites flanking a critical exon of Smo, allowing Cre recombinase dependent deletion in specific tissues or at specific timepoints.
Modifications available
Jump to catalog backed pages for Smo organized by modification type. Each URL is indexable and matches common search patterns.