Sost Knockin mouse models
Sost is the mouse ortholog of human SOST and encodes sclerostin, an osteocyte-derived, secreted cysteine-knot protein of the DAN family that binds the first beta-propeller of LRP5 and LRP6, using LRP4 as a presenting platform, to block Wnt, Frizzled, and LRP coreceptor assembly and suppress beta-catenin signaling in osteoblasts, with a secondary reported BMP-antagonist activity. Mechanical loading downregulates it, and human loss of function causes sclerosteosis or, through deletion of the ECR5 enhancer roughly 35 kilobases downstream, van Buchem disease, both high bone mass conditions that validated antibody blockade. This makes the allele fork unusually crisp: osteocyte-restricted conditional nulls using Dmp1-Cre test cell of origin and adult loading responses, ECR5 enhancer deletion models the human regulatory disease, and humanizing the LRP-binding loop enables direct antibody pharmacology. Sostdc1 shares the same LRP-binding mode, so consider compound alleles when dental or renal endpoints accompany skeletal readouts.
Targeting Sost preserves positional context compared with viral or BAC approaches. That helps when you quantify expression or map chromatin.
What Sost Knockin mouse models are available?
ingenious targeting laboratory offers 1 distinct Sost knockin catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Sost-CreERT2 | Knockin | Cre/Dre Toolbox of Mice, disease model mice | sperm cryopreservation | KI 215085 | Inquire |
Why this approach
Knockin and humanized formats keep regulatory context at the endogenous locus. For your target tissue focused programs, that matters when expression timing, splice isoforms, or allele dosage drive biology. Random integration transgenics can still help, but targeted alleles usually give cleaner pharmacology readouts.
Pricing and quotes
The Sost Knockin lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Sost allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Sost Knockin mouse models are available?
When Sost Knockin lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Sost knockout embryonic lethal in mice?
It depends on background and allele design. Some Sost germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Sost animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Sost?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.